Murayama Chiaki, Yamasaki Eiki, Miyamoto Akio, Shimizu Takashi
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
Biochem Biophys Res Commun. 2015 Jul 10;462(4):415-9. doi: 10.1016/j.bbrc.2015.05.005. Epub 2015 May 12.
Ovarian follicular cysts are one of the most common causes of reproductive failure in mammals. A comparative gene expression approach may aid in elucidating the causes of ovarian cyst disease. In the present study, the differential display technique was used to identify mRNA sequences that accumulate preferentially in theca cells of bovine cystic follicles. Dedicator of cytokinesis 6 (Dock6) expression was observed in the theca cells of cystic follicles. Small interfering RNA (siRNA) knockdown of Dock6 increased progesterone (P4) production and StAR expression in theca cells of high-estrogen follicular cysts, but did not affect androstenedione (A4) production. We propose that Dock6 may be a marker associated with the development of follicular cysts. Additionally, Dock6 may be involved in the development of cystic follicles by suppressing P4 production rather than increasing A4 production in theca cells.
卵巢滤泡囊肿是哺乳动物生殖失败的最常见原因之一。比较基因表达方法可能有助于阐明卵巢囊肿疾病的病因。在本研究中，采用差异显示技术来鉴定在牛囊性卵泡的膜细胞中优先积累的mRNA序列。在囊性卵泡的膜细胞中观察到细胞分裂素6（Dock6）的表达。小干扰RNA（siRNA）敲低Dock6可增加高雌激素卵泡囊肿膜细胞中孕酮（P4）的产生和类固醇生成急性调节蛋白（StAR）的表达，但不影响雄烯二酮（A4）的产生。我们认为Dock6可能是与滤泡囊肿发展相关的标志物。此外，Dock6可能通过抑制膜细胞中P4的产生而非增加A4的产生参与囊性卵泡的发展。
