Ono Tetsushi, Takagi Mitsuhiro, Kawashima Chiho, Wijayagunawardane Missaka P B, Vos Peter L A M, Taniguchi Masayasu, Tanihara Fuminori, Otoi Takeshige
Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Japan.
Laboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Front Vet Sci. 2018 Jun 29;5:130. doi: 10.3389/fvets.2018.00130. eCollection 2018.
The objective of this study was to determine the effects of different intramuscular dosages of human chorionic gonadotropin (hCG) on ovarian follicular development of dairy cows diagnosed with refractory cystic ovarian follicles (COFs). Cows diagnosed with COFs (≥25 mm in diameter) were allocated to four treatment groups: hCG-1 ( = 3), a single dose of 4,500 IU on day 1; hCG-2 ( = 3), 2,250 IU on days 1 and 3; hCG-3 ( = 3), 1,500 IU on days 1, 3, and 5; and hCG-C ( = 3) received saline on day 1. Blood sampling and ovarian ultrasonographic (US) examinations were performed on days 1, 3, 5, 7, and 14. A progesterone (P4) value < 1 ng/ml was used as an indicator of absence of a functional CL. A significant increase ( < 0.05) in the number of follicles < 4 mm in diameter was observed in the hCG-2 group on day 5. Additionally, there was a significant difference in the number of follicles < 4 mm ( < 0.05) between both the hCG-2 and hCG-3 groups compared to the hCG-C group on day 5, and a tendency ( = 0.08) toward a difference in the number of 5-9 mm follicles in groups hCG-3, hCG-2, and hCG-1, compared with the hCG-C group on day 7. The proportion of cows on days 7 and 14 with P4 > 1 ng/ml was 100% (3/3) and 100% (3/3) in group hCG-1; 100% (3/3) and 67% (2/3) in group hCG-2; 67% (2/3) and 100% (3/3) in group hCG-3; and 33% (1/3) and 33% (1/3) in group hCG-C, respectively. Strong tendencies of P4 increases in group hCG-1 ( = 0.054) and hCG-2 ( = 0.051) were measured after hCG administration. Additionally, P4 values tended to be higher ( = 0.07) for group hCG-1 compared to group hCG-C on day 5. The preliminary findings of this study suggest that multiple smaller doses of hCG might be equally effective as a single large dose of hCG in modulating ovarian follicular development in dairy cows with COFs.
本研究的目的是确定不同肌肉注射剂量的人绒毛膜促性腺激素（hCG）对诊断为难治性囊性卵巢卵泡（COF）的奶牛卵巢卵泡发育的影响。将诊断为COF（直径≥25 mm）的奶牛分为四个治疗组：hCG-1组（n = 3），第1天单次注射4500 IU；hCG-2组（n = 3），第1天和第3天各注射2250 IU；hCG-3组（n = 3），第1天、第3天和第5天各注射1500 IU；hCG-C组（n = 3）在第1天接受生理盐水注射。在第1、3、5、7和14天进行采血和卵巢超声（US）检查。孕酮（P4）值<1 ng/ml用作无功能性黄体（CL）的指标。在第5天，hCG-2组直径<4 mm的卵泡数量显著增加（P<0.05）。此外，在第5天，hCG-2组和hCG-3组直径<4 mm的卵泡数量与hCG-C组相比均存在显著差异（P<0.05），在第7天，hCG-3组、hCG-2组和hCG-1组5-9 mm卵泡数量与hCG-C组相比有差异趋势（P = 0.08）。在第7天和第14天，hCG-1组P4>1 ng/ml的奶牛比例分别为100%（3/3）和100%（3/3）；hCG-2组为100%（3/3）和67%（2/3）；hCG-3组为67%（2/3）和100%（3/3）；hCG-C组为33%（1/3）和33%（1/3）。在注射hCG后，测量到hCG-1组（P = 0.054）和hCG-2组（P = 0.051）的P4有强烈的升高趋势。此外，在第5天，hCG-1组的P4值与hCG-C组相比有更高的趋势（P = 0.07）。本研究的初步结果表明，在调节患有COF的奶牛卵巢卵泡发育方面，多次小剂量的hCG可能与单次大剂量的hCG同样有效。
