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EcoRII限制性核酸内切酶基因的核苷酸序列。

Nucleotide sequence of the EcoRII restriction endonuclease gene.

作者信息

Kossykh V, Repyk A, Kaliman A, Buryanov Y

机构信息

Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R. Academy of Sciences, Pushchino.

出版信息

Biochim Biophys Acta. 1989 Dec 22;1009(3):290-2. doi: 10.1016/0167-4781(89)90117-6.

Abstract

The nucleotide sequence of a 1394 basepair (bp) DNA fragment containing the EcoRII restriction endonuclease (R.EcoRII) gene was determined. The endonuclease gene is 1206 bp in length (predicted 402 amino acids (aa) and Mr = 45 178) and is separated by 33 bp from the EcoRII modification methylase (M.EcoRII) gene. The EcoRII restriction-modification system has a tail-to-tail organization of the two genes.

摘要

测定了包含EcoRII限制性内切核酸酶(R.EcoRII)基因的1394碱基对(bp)DNA片段的核苷酸序列。该内切核酸酶基因长度为1206 bp(预测为402个氨基酸(aa),Mr = 45178),与EcoRII甲基化修饰酶(M.EcoRII)基因相隔33 bp。EcoRII限制修饰系统的这两个基因呈尾对尾排列。

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