• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

EcoRII限制性核酸内切酶基因的核苷酸序列。

Nucleotide sequence of the EcoRII restriction endonuclease gene.

作者信息

Kossykh V, Repyk A, Kaliman A, Buryanov Y

机构信息

Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R. Academy of Sciences, Pushchino.

出版信息

Biochim Biophys Acta. 1989 Dec 22;1009(3):290-2. doi: 10.1016/0167-4781(89)90117-6.

DOI:10.1016/0167-4781(89)90117-6
PMID:2597679
Abstract

The nucleotide sequence of a 1394 basepair (bp) DNA fragment containing the EcoRII restriction endonuclease (R.EcoRII) gene was determined. The endonuclease gene is 1206 bp in length (predicted 402 amino acids (aa) and Mr = 45 178) and is separated by 33 bp from the EcoRII modification methylase (M.EcoRII) gene. The EcoRII restriction-modification system has a tail-to-tail organization of the two genes.

摘要

测定了包含EcoRII限制性内切核酸酶(R.EcoRII)基因的1394碱基对(bp)DNA片段的核苷酸序列。该内切核酸酶基因长度为1206 bp(预测为402个氨基酸(aa),Mr = 45178),与EcoRII甲基化修饰酶(M.EcoRII)基因相隔33 bp。EcoRII限制修饰系统的这两个基因呈尾对尾排列。

相似文献

1
Nucleotide sequence of the EcoRII restriction endonuclease gene.EcoRII限制性核酸内切酶基因的核苷酸序列。
Biochim Biophys Acta. 1989 Dec 22;1009(3):290-2. doi: 10.1016/0167-4781(89)90117-6.
2
Primary sequence of the EcoRII endonuclease and properties of its fusions with beta-galactosidase.EcoRII核酸内切酶的一级序列及其与β-半乳糖苷酶融合体的特性
J Biol Chem. 1990 Jan 15;265(2):767-73.
3
A study of the Asp110-Glu112 region of EcoRII restriction endonuclease by site-directed mutagenesis.
Biochemistry (Mosc). 2000 Sep;65(9):1006-10.
4
Sequence motifs common to the EcoRII restriction endonuclease and the proposed sequence specificity domain of three DNA-[cytosine-C5] methyltransferases.EcoRII限制内切酶与三种DNA-[胞嘧啶-C5]甲基转移酶的拟序列特异性结构域共有的序列基序。
Gene. 1993 Mar 15;125(1):65-8. doi: 10.1016/0378-1119(93)90746-p.
5
Identification of a DNA restriction-modification system in Pectobacterium carotovorum strains isolated from Poland.从波兰分离的胡萝卜软腐果胶杆菌菌株中DNA限制修饰系统的鉴定。
J Appl Microbiol. 2006 Feb;100(2):343-51. doi: 10.1111/j.1365-2672.2005.02766.x.
6
Expression and deletion analysis of EcoRII endonuclease and methylase gene.EcoRII核酸内切酶和甲基化酶基因的表达及缺失分析
Chin Med Sci J. 2001 Dec;16(4):200-3.
7
The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes.福克I型限制修饰系统。I. 限制与修饰基因的组织及核苷酸序列
J Biol Chem. 1989 Apr 5;264(10):5751-6.
8
Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes.StsI 限制修饰基因的克隆与序列分析:与 FokI 限制修饰酶存在同源性
Nucleic Acids Res. 1992 Aug 25;20(16):4167-72. doi: 10.1093/nar/20.16.4167.
9
[Interaction of restriction and modification enzyme EcoRII with synthetic DNA fragments. VII. The study of complex-formation of endonuclease EcoRII with substrates containing natural and modified recognition sites].[限制性内切酶EcoRII与合成DNA片段的相互作用。VII. 核酸内切酶EcoRII与含有天然和修饰识别位点的底物形成复合物的研究]
Mol Biol (Mosk). 1986 Sep-Oct;20(5):1329-36.
10
[Primary structure of the gene of restriction endonuclease EcoRII].[限制性内切酶EcoRII基因的一级结构]
Dokl Akad Nauk SSSR. 1989;308(6):1497-9.

引用本文的文献

1
Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides.鉴定具有切割单纯疱疹病毒2型基因组潜在能力的限制性内切酶:生物合成型与活重组杀微生物剂的内在潜力。
Theor Biol Med Model. 2008 Aug 7;5:18. doi: 10.1186/1742-4682-5-18.
2
Evidence for horizontal transfer of the EcoT38I restriction-modification gene to chromosomal DNA by the P2 phage and diversity of defective P2 prophages in Escherichia coli TH38 strains.P2噬菌体介导EcoT38I限制修饰基因水平转移至染色体DNA的证据以及大肠杆菌TH38菌株中缺陷型P2原噬菌体的多样性
J Bacteriol. 2003 Apr;185(7):2296-305. doi: 10.1128/JB.185.7.2296-2305.2003.
3
Structure and function of type II restriction endonucleases.
II型限制性核酸内切酶的结构与功能。
Nucleic Acids Res. 2001 Sep 15;29(18):3705-27. doi: 10.1093/nar/29.18.3705.
4
Evidence of horizontal transfer of the EcoO109I restriction-modification gene to Escherichia coli chromosomal DNA.EcoO109I限制修饰基因向大肠杆菌染色体DNA水平转移的证据。
J Bacteriol. 1999 Nov;181(21):6822-7. doi: 10.1128/JB.181.21.6822-6827.1999.
5
New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
Nucleic Acids Res. 1990 Mar 11;18(5):1321-30. doi: 10.1093/nar/18.5.1321.
6
Organization of restriction-modification systems.限制-修饰系统的组织
Nucleic Acids Res. 1991 May 25;19(10):2539-66. doi: 10.1093/nar/19.10.2539.
7
Activation of restriction endonuclease EcoRII does not depend on the cleavage of stimulator DNA.限制性内切酶EcoRII的激活并不依赖于刺激物DNA的切割。
Nucleic Acids Res. 1991 Oct 11;19(19):5139-42. doi: 10.1093/nar/19.19.5139.