Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Institute of Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Scheldeweg 68, B-9090 Melle, Belgium.
Food Chem. 2015 Nov 15;187:120-9. doi: 10.1016/j.foodchem.2015.04.066. Epub 2015 Apr 22.
Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory reproducibility (RSD<10.5%), recovery (87-97%) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).
公猪异味是在加热公猪肉或脂肪时产生的一种异味。一种能够同时检测血液中三种化合物（雄甾酮、粪臭素和吲哚）的方法具有很大的优势，因为它可以监测饲养策略的影响。因此，优化并验证了一种用于检测血浆或血清中这些化合物的 UHPLC-HR-Orbitrap-MS 分析方法。样品预处理包括用二乙醚提取，然后进行离心过滤（30 kDa）。三种化合物的检测限和定量限分别在 0.5 到 1 μg L(-1) 和 2 到 3 μg L(-1)之间。此外，还记录了极好的重复性（RSD < 7.6%）、实验室内再现性（RSD<10.5%）、回收率（87-97%）和线性（R(2)>0.99）。粪臭素和雄甾酮在血清/血浆和脂肪水平之间的相关性呈正相关（r(血清) = 0.39 和 r(血浆) = 0.84）和（r(血清) = 0.73-0.78 和 r(血浆) = 0.32-0.80）。
