Afe Ayoola Ebenezer, Shen Zhao-Ji, Guo Xiaorong, Xing Weihai, Li Kui, Zhou Rong
The State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Food Chem X. 2024 Jul 22;24:101686. doi: 10.1016/j.fochx.2024.101686. eCollection 2024 Dec 30.
This study presents a sensitive and reproducible mass spectrometry method for quantifying skatole in porcine adipose tissue, muscle, and serum samples applicable for abattoirs and laboratories. Leveraging gas chromatography-high-resolution Orbitrap microscopy and microwave-assisted liquefication of the adipose tissue, the method demonstrates robust performance across key parameters. Impressive linearity (R) values of 0.9999 and 0.9996 for adipose tissue and serum, respectively. Notably, the method exhibits a low Limit of Detection (LoD) of 0.5 ppb for adipose tissue and 0.9 ppb for serum, with corresponding Limits of Quantification (LoQ) at 1.65 ppb and 3.04 ppb, respectively. The method showed significant reproducibility of 5.9% and repeatability (RSD%) of 8.78% for adipose tissue and 4.08% for serum, with recovery rates of 90% and 87%, respectively. This streamlined method offers promising, effective quantification of boar taint compounds, emphasizing its sensitivity and reproducibility.
本研究提出了一种灵敏且可重复的质谱分析法，用于定量猪脂肪组织、肌肉和血清样本中的粪臭素，适用于屠宰场和实验室。该方法利用气相色谱-高分辨率轨道阱显微镜以及脂肪组织的微波辅助液化技术，在关键参数方面展现出稳健的性能。脂肪组织和血清的线性（R）值分别令人印象深刻，达到0.9999和0.9996。值得注意的是，该方法对脂肪组织的检测限（LoD）低至0.5 ppb，对血清的检测限为0.9 ppb，相应的定量限（LoQ）分别为1.65 ppb和3.04 ppb。该方法对脂肪组织的重现性显著，相对标准偏差（RSD%）为5.9%，重复性为8.78%，对血清的重复性为4.08%，回收率分别为90%和87%。这种简化的方法为公猪异味化合物提供了有前景、有效的定量分析，突出了其灵敏度和可重复性。
