Zhang Yangyang, Zeng Lizhen, Yang Jiali, Zheng Xiaodong, Yu Ting
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, People's Republic of China.
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, People's Republic of China.
Food Chem. 2015 Nov 15;187:210-7. doi: 10.1016/j.foodchem.2015.04.100. Epub 2015 Apr 23.
This study demonstrated the inhibitory effect of 6-benzylaminopurine (BAP), the first generation synthetic cytokinin, on the invasion of Monilinia fructicola in peach fruit and the possible mechanism involved for the first time. Our results suggested that BAP treatment had a 63% lower disease incidence and approximately 10 times lower lesion diameter compared to the control throughout the incubation period. In vitro BAP showed a direct inhibitory effect on M. fructicola spore germination. BAP could prevent fruit texture deterioration and protect the cell membrane from oxidative stress, while no adverse effects were observed on fruit quality maintenance. Analysis of defense-related enzymes activities indicated that the use of BAP induced higher specific polyphenol oxidase and peroxidase activities which triggered stronger host defensive responses. Thus, our results verified the proposed mechanism of BAP in controlling M. fructicola by direct inhibitory effect, delay peach senescence and activation of defensive enzymes.
本研究首次证明了第一代合成细胞分裂素6-苄基腺嘌呤（BAP）对桃果实中褐腐病菌（Monilinia fructicola）侵染的抑制作用及其可能的作用机制。我们的结果表明，在整个培养期内，与对照相比，BAP处理的发病率降低了63%，病斑直径降低了约10倍。在体外，BAP对褐腐病菌孢子萌发具有直接抑制作用。BAP可以防止果实质地恶化，并保护细胞膜免受氧化应激，同时在维持果实品质方面未观察到不利影响。对防御相关酶活性的分析表明，使用BAP可诱导更高的特异性多酚氧化酶和过氧化物酶活性，从而引发更强的宿主防御反应。因此，我们的结果证实了BAP通过直接抑制作用、延缓桃衰老和激活防御酶来控制褐腐病菌的作用机制。
