Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University, POB 269, 54124, Thessaloniki, Greece.
Institute of Soil and Water Resources, ELGO-Demeter Thermi, Thessaloniki, Greece.
Sci Rep. 2020 May 8;10(1):7807. doi: 10.1038/s41598-020-64864-x.
Brown rot, caused by Monilinia spp., is a major peach disease worldwide. In this study, the response of peach cultivars Royal Glory (RG) and Rich Lady (RL) to infection by Monilinia fructicola or Monilinia laxa, was characterized. Phenotypic data, after artificial inoculations, revealed that 'RL' was relatively susceptible whereas 'RG' was moderately resistant to Monilinia spp. Comparative proteomic analysis identified mesocarp proteins of the 2 cultivars whose accumulation were altered by the 2 Monilinia species. Functional analysis indicated that pathogen-affected proteins in 'RG' were mainly involved in energy and metabolism, while, differentially accumulated proteins by the pathogen presence in 'RL' were involved in disease/defense and metabolism. A higher number of proteins was differentiated in 'RG' fruit compared to 'RL'. Upon Monilinia spp. infection, various proteins were-down accumulated in 'RL' fruit. Protein identification by mass spectrometric analysis revealed that several defense-related proteins including thaumatin, formate dehydrogenase, S-formylglutathione hydrolase, CBS domain-containing protein, HSP70, and glutathione S-transferase were up-accumulated in 'RG' fruit following inoculation. The expression profile of selected defense-related genes, such as major latex allergen, 1-aminocyclopropane-1-carboxylate deaminase and UDP-glycoltransferase was assessed by RT-PCR. This is the first study deciphering differential regulations of peach fruit proteome upon Monilinia infection elucidating resistance responses.
桃褐腐病由 Monilinia spp.引起，是一种世界性的桃病害。在本研究中，对桃品种 Royal Glory (RG) 和 Rich Lady (RL) 感染 Monilinia fructicola 或 Monilinia laxa 的反应进行了研究。人工接种后的表型数据表明，‘RL’相对易感，而‘RG’对 Monilinia spp.中度抗性。比较蛋白质组学分析鉴定了 2 个品种的中果皮蛋白，这 2 个品种的蛋白积累受 2 种 Monilinia 物种的影响。功能分析表明，受病原菌影响的‘RG’果实中的蛋白主要参与能量和代谢，而病原菌存在时‘RL’果实中差异积累的蛋白则参与疾病/防御和代谢。与‘RL’果实相比，‘RG’果实中分化的蛋白数量更多。在 Monilinia spp.感染后，‘RL’果实中的多种蛋白下调。通过质谱分析鉴定蛋白质发现，几种防御相关蛋白包括几丁质酶、甲酸脱氢酶、S-甲酰谷胱甘肽水解酶、CBS 结构域蛋白、HSP70 和谷胱甘肽 S-转移酶在接种后在‘RG’果实中上调积累。通过 RT-PCR 评估了几丁质酶、1-氨基环丙烷-1-羧酸脱氨酶和 UDP-糖基转移酶等选定防御相关基因的表达谱。这是首次研究阐明桃果实蛋白组在 Monilinia 感染下的差异调控，揭示了抗性反应。
