Liberto Melina G Di, Seimandi Gisela M, Fernández Laura N, Ruiz Verónica E, Svetaz Laura A, Derita Marcos G
Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario S2002LRK, Argentina.
ICiAgro Litoral, Universidad Nacional del Litoral, CONICET, Facultad de Ciencias Agrarias, Kreder 2805, Esperanza 3080HOF, Argentina.
Plants (Basel). 2021 Feb 24;10(3):425. doi: 10.3390/plants10030425.
(Polygonaceae) is a perennial herb that grows in the central area of Argentina and it is commonly used by native populations to heal infected wounds and other conditions related to fungal infections. In this article, we explored the antifungal activity of its ethyl acetate extract against a panel of three fruit phytopathogenic fungi including: , and . The sesquiterpenes isolated from the extract were also evaluated against these strains, demonstrating that the dialdehyde polygodial was the responsible for this activity. In order to encourage the use of the extract rather than the pure compound, we displayed assays using fresh oranges and peaches inoculated with and , respectively, and subsequently treated by immersion with an extract solution of 250 and 62.5 µg/mL, respectively. There were no statistically significant differences between the treatments with commercial fungicides and the extract over the control of both fruit rots. The concentration of the active compound present in the extract used on fruit experiments was determined by Gas Chromatography-Mass Spectroscopy. Finally, cytotoxicity evaluation against Huh7 cells showed that extract was less cytotoxic than the commercial fungicides at the assayed concentrations. After these findings we could conclude that a chemically characterized extract of should be further developed to treat fungal diseases in fruits from an agro-ecological model.
蓼科植物是一种多年生草本植物，生长在阿根廷中部地区，当地居民常用它来治疗感染伤口和其他与真菌感染相关的病症。在本文中，我们探究了其乙酸乙酯提取物对三种水果植物病原真菌（包括[具体真菌名称1]、[具体真菌名称2]和[具体真菌名称3]）的抗真菌活性。从提取物中分离出的倍半萜类化合物也针对这些菌株进行了评估，结果表明二醛类化合物多戈dial是具有这种活性的成分。为了鼓励使用提取物而非纯化合物，我们分别对接种了[具体真菌名称1]和[具体真菌名称2]的新鲜橙子和桃子进行了试验，随后分别用浓度为250和62.5μg/mL的提取物溶液浸泡处理。在控制两种水果腐烂方面，商业杀菌剂处理与提取物处理之间与对照相比没有统计学上的显著差异。通过气相色谱 - 质谱联用仪测定了水果实验中所用提取物中活性化合物的浓度。最后，针对Huh7细胞的细胞毒性评估表明，在测定浓度下，[植物名称]提取物的细胞毒性低于商业杀菌剂。基于这些发现，我们可以得出结论，应进一步开发一种经过化学表征 的提取物，以从农业生态模式治疗水果中的真菌病害。
