Torlakovic E E, Brynes R K, Hyjek E, Lee S-H, Kreipe H, Kremer M, McKenna R, Sadahira Y, Tzankov A, Reis M, Porwit A
Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada.
Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Int J Lab Hematol. 2015 Aug;37(4):431-49. doi: 10.1111/ijlh.12365. Epub 2015 May 15.
Bone marrow (BM) tissue biopsy evaluation, including trephine biopsy and clot section, is an integral part of BM investigation and is often followed by ancillary studies, in particular immunohistochemistry (IHC). IHC provides in situ coupling of morphological assessment and immunophenotype. The number of different IHC tests that can be applied to BM trephine biopsies and the number of indications for IHC testing is increasing concurrently with the development of flow cytometry and molecular diagnostic methods. An international Working Party for the Standardization of Bone Marrow IHC was formed by the International Council for Standardization in Hematology (ICSH) to prepare a set of guidelines for the standardization of BM IHC based on currently available published evidence and modern understanding of quality assurance principles as applied to IHC in general. The guidelines were discussed at the ICSH General Assemblies and reviewed by an international panel of experts to achieve further consensus and represent further development of the previously published ICSH guidelines for the standardization of BM specimens handling and reports.
骨髓（BM）组织活检评估，包括环钻活检和凝块切片，是骨髓检查不可或缺的一部分，之后通常还会进行辅助研究，尤其是免疫组织化学（IHC）。免疫组织化学可在原位将形态学评估与免疫表型相结合。随着流式细胞术和分子诊断方法的发展，可应用于骨髓环钻活检的不同免疫组织化学检测数量以及免疫组织化学检测的适应证数量都在不断增加。国际血液学标准化委员会（ICSH）成立了一个骨髓免疫组织化学标准化国际工作组，旨在根据目前已发表的证据以及对一般免疫组织化学应用的质量保证原则的现代理解，制定一套骨髓免疫组织化学标准化指南。这些指南在国际血液学标准化委员会大会上进行了讨论，并由一个国际专家小组进行了审查，以达成进一步的共识，并代表对国际血液学标准化委员会先前发布的骨髓标本处理和报告标准化指南的进一步发展。
