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DIANA-miRPath v3.0：借助实验支持解读微小RNA功能。

DIANA-miRPath v3.0: deciphering microRNA function with experimental support.

作者信息

Vlachos Ioannis S, Zagganas Konstantinos, Paraskevopoulou Maria D, Georgakilas Georgios, Karagkouni Dimitra, Vergoulis Thanasis, Dalamagas Theodore, Hatzigeorgiou Artemis G

机构信息

DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, 382 21 Volos, Greece Laboratory for Experimental Surgery and Surgical Research 'N.S. Christeas', Medical School of Athens, University of Athens, 11527 Athens, Greece

'Athena' Research and Innovation Center, 11524 Athens, Greece University of Peloponnisos, Department of Informatics and Telecommunications, 22100 Tripoli, Greece.

出版信息

Nucleic Acids Res. 2015 Jul 1;43(W1):W460-6. doi: 10.1093/nar/gkv403. Epub 2015 May 14.

DOI:10.1093/nar/gkv403
PMID:25977294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4489228/
Abstract

The functional characterization of miRNAs is still an open challenge. Here, we present DIANA-miRPath v3.0 (http://www.microrna.gr/miRPathv3) an online software suite dedicated to the assessment of miRNA regulatory roles and the identification of controlled pathways. The new miRPath web server renders possible the functional annotation of one or more miRNAs using standard (hypergeometric distributions), unbiased empirical distributions and/or meta-analysis statistics. DIANA-miRPath v3.0 database and functionality have been significantly extended to support all analyses for KEGG molecular pathways, as well as multiple slices of Gene Ontology (GO) in seven species (Homo sapiens, Mus musculus, Rattus norvegicus, Drosophila melanogaster, Caenorhabditis elegans, Gallus gallus and Danio rerio). Importantly, more than 600 000 experimentally supported miRNA targets from DIANA-TarBase v7.0 have been incorporated into the new schema. Users of DIANA-miRPath v3.0 can harness this wealth of information and substitute or combine the available in silico predicted targets from DIANA-microT-CDS and/or TargetScan v6.2 with high quality experimentally supported interactions. A unique feature of DIANA-miRPath v3.0 is its redesigned Reverse Search module, which enables users to identify and visualize miRNAs significantly controlling selected pathways or belonging to specific GO categories based on in silico or experimental data. DIANA-miRPath v3.0 is freely available to all users without any login requirement.

摘要

微小RNA（miRNA）的功能表征仍是一个有待解决的挑战。在此，我们展示了DIANA-miRPath v3.0（http://www.microrna.gr/miRPathv3），这是一个在线软件套件，致力于评估miRNA的调控作用并识别受其控制的通路。新的miRPath网络服务器能够使用标准（超几何分布）、无偏经验分布和/或荟萃分析统计数据对一个或多个miRNA进行功能注释。DIANA-miRPath v3.0的数据库和功能已得到显著扩展，以支持对KEGG分子通路以及七个物种（智人、小家鼠、褐家鼠、黑腹果蝇、秀丽隐杆线虫、原鸡和斑马鱼）的多个基因本体论（GO）切片进行的所有分析。重要的是，来自DIANA-TarBase v7.0的超过60万个经实验支持的miRNA靶标已被纳入新架构。DIANA-miRPath v3.0的用户可以利用这些丰富的信息，用高质量的实验支持的相互作用替代或组合来自DIANA-microT-CDS和/或TargetScan v6.2的现有计算机预测靶标。DIANA-miRPath v3.0的一个独特功能是其重新设计的反向搜索模块，该模块使用户能够根据计算机模拟或实验数据识别并可视化显著控制选定通路或属于特定GO类别的miRNA。DIANA-miRPath v3.0对所有用户免费开放，无需任何登录要求。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/fe6096a281e6/gkv403fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/48ad4e7bbb99/gkv403fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/09308e757c49/gkv403fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/fe6096a281e6/gkv403fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/48ad4e7bbb99/gkv403fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/09308e757c49/gkv403fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efa/4489228/fe6096a281e6/gkv403fig3.jpg

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