神经嵴来源的牙髓干细胞的富集与雪旺细胞分化

Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells.

作者信息

Al-Zer Heba, Apel Christian, Heiland Max, Friedrich Reinhard E, Jung Ole, Kroeger Nadja, Eichhorn Wolfgang, Smeets Ralf

机构信息

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, University of Hamburg, Hamburg, Germany

Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of the RWTH Aachen University, Aachen, Germany.

出版信息

In Vivo. 2015 May-Jun;29(3):319-26.

PMID:25977377

Abstract

BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.

MATERIALS AND METHODS

Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.

RESULTS

The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.

CONCLUSION

Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.

摘要

背景/目的：正如先前研究中所描述的，成年人类牙髓中可发现神经嵴干细胞（NCSCs）。本研究调查了上述细胞的富集和诱导分化方法。

材料与方法

从4名患者的智齿中提取牙髓，随后将其作为外植体培养在涂有纤连蛋白的培养板上，培养基为添加了B27、碱性成纤维细胞生长因子（bFGF）、表皮生长因子（EGF）、胰岛素、L-谷氨酰胺和神经调节蛋白-β1的神经基础培养基。然后通过免疫荧光对细胞进行表征，同时通过尝试将细胞诱导分化为不同谱系，即成骨细胞、黑素细胞和神经胶质细胞，来测试其分化潜能。