Go Hiroe, Ryuk Jin Ah, Lee Hye Won, Park In Sil, Kil Ki-Jung, Park Sunmin, Kim Dong Il, Ko Byoung Seob
Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
College of Oriental Medicine, Joongbu University, 201 Daehak-Ro, Chubu-Myeon, Geumsan-Gun, ChungNam 312-702, Republic of Korea.
Evid Based Complement Alternat Med. 2015;2015:321407. doi: 10.1155/2015/321407. Epub 2015 Apr 22.
The present study was conducted to investigate the effect of Sagunja-tang on the lipid related disease in a rat model of menopausal hyperlipidemia and lipid accumulation in methyl-β-cyclodextrin-induced HepG2 cells. In in vivo study using menopausal hyperlipidemia rats, Sagunja-tang reduced retroperitoneal and perirenal fat, serum lipids, atherogenic index, cardiac risk factor, media thickness, and nonalcoholic steatohepatitis score, when compared to menopausal hyperlipidemia control rats. In HepG2 cells, Sagunja-tang significantly decreased the lipid accumulation, total cholesterol levels, and low-density/very-low-density lipoprotein levels. Moreover, Sagunja-tang reversed the methyl-β-cyclodextrin-induced decrease in the protein levels of critical molecule involved in cholesterol synthesis, sterol regulatory element binding protein-2, and low-density lipoprotein receptor and inhibited protein levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase as well as activity. Phosphorylation level of AMP-activated protein kinase was stimulated by Sagunja-tang. These results suggest that Sagunja-tang has effect on inhibiting hepatic lipid accumulation through regulation of cholesterol synthesis and AMPK activity in vitro. These observations support the idea that Sagunja-tang is bioavailable both in vivo and in vitro and could be developed as a preventive and therapeutic agent of hyperlipidemia in postmenopausal females.
本研究旨在探讨四物降血脂汤对绝经后高脂血症大鼠模型脂质相关疾病以及甲基-β-环糊精诱导的HepG2细胞脂质蓄积的影响。在使用绝经后高脂血症大鼠的体内研究中，与绝经后高脂血症对照大鼠相比，四物降血脂汤减少了大鼠腹膜后和肾周脂肪、血清脂质、致动脉粥样硬化指数、心脏危险因素、血管中层厚度以及非酒精性脂肪性肝炎评分。在HepG2细胞中，四物降血脂汤显著降低了脂质蓄积、总胆固醇水平以及低密度/极低密度脂蛋白水平。此外，四物降血脂汤逆转了甲基-β-环糊精诱导的胆固醇合成关键分子、固醇调节元件结合蛋白-2以及低密度脂蛋白受体蛋白水平的降低，并抑制了3-羟基-3-甲基戊二酰辅酶A还原酶的蛋白水平及其活性。四物降血脂汤可刺激AMP活化蛋白激酶的磷酸化水平。这些结果表明，四物降血脂汤在体外可通过调节胆固醇合成和AMPK活性来抑制肝脏脂质蓄积。这些观察结果支持了四物降血脂汤在体内和体外均具有生物活性，并且可开发成为绝经后女性高脂血症预防和治疗药物的观点。
