MRC Centre for Developmental Neurobiology, King's College London, 4th Floor New Hunt's House , London SE1 1UL, UK.
Drug Discovery Unit, Wolfson Centre for Age Related Disease, King's College London, Guy's Campus , London SE1 1UL, UK.
Sci Data. 2014 Jul 22;1:140014. doi: 10.1038/sdata.2014.14. eCollection 2014.
The anteroposterior compartments of the developing hindbrain (rhombomeres [r]) are normally patterned by the combinatorial action of distinct Hox genes. Using Affymetrix GeneChips to define the repertoire of genes regulated in each rhombomere, we have performed a systematic survey of the transcriptional status of individual segments of the developing chick hindbrain (r1-5) at a key stage of early development (HH11) and identified hundreds of previously un-described genes expressed in this region. For comparative purposes, we have also included the adjacent region of the embryonic midbrain (m) in our dataset. In summary, six different embryonic brain regions (m, r1, r2, r3, r4 & r5) are represented by biological duplicates to give a raw dataset comprised of 12 individual Affymetrix GeneChip Cel and CHP files. These data give an opportunity to assess the genome-wide complexity of gene expression during patterning of the chick developing midbrain and hindbrain, and may be relevant to extending our understanding of the genes regulated by Hox family transcription factors.
发育中后脑（神经节[rhombomeres（r）]的前后腔室通常通过不同 Hox 基因的组合作用进行模式化。我们使用 Affymetrix GeneChips 来定义每个神经节中受调节的基因组合，在早期发育的关键阶段（HH11）对发育中的鸡后脑（r1-5）的单个节段的转录状态进行了系统调查，并鉴定了数百个以前未在该区域表达的基因。为了进行比较，我们还将胚胎中脑（m）的相邻区域包括在我们的数据集内。总之，通过生物重复代表了六个不同的胚胎脑区域（m、r1、r2、r3、r4 和 r5），以提供由 12 个单独的 Affymetrix GeneChip Cel 和 CHP 文件组成的原始数据集。这些数据提供了评估在小鸡发育中的中脑和后脑模式化过程中整个基因组范围内基因表达复杂性的机会，并且可能与扩展我们对 Hox 家族转录因子调节的基因的理解有关。
