Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 1760 Haygood Drive, HSRB E170 , Atlanta, Georgia 30322, USA.
School of Applied Biosciences, Kyungpook National University , Daegu 702-701, South Korea.
Sci Data. 2014 Oct 28;1:140039. doi: 10.1038/sdata.2014.39. eCollection 2014.
Atherosclerosis preferentially occurs in arterial regions exposed to disturbed blood flow (d-flow), in part, due to alterations in gene expression in the endothelium. While numerous in vitro studies have shown how anti-atherogenic flow and pro-atherogenic flow differently regulate gene expression of cultured endothelial cells, similar in vivo studies have been scarce. Recently, we developed a mouse model of atherosclerosis that rapidly develops robust atherosclerosis by partially ligating the left carotid artery (LCA) branches, while using the contralateral right carotid (RCA) as control. We also developed a novel method to collect endothelial-enriched RNAs from the carotids of these animals, which enabled us to perform genome-wide expression analyses of mRNAs and miRNAs in the arterial endothelium exposed to either d-flow or s-flow. These microarray results were used to identify novel mechanosensitive genes such as DNA methyltransferase-1 and miR-712 that play key roles in atherosclerosis. Here, we report these endothelial mRNA and miRNA expression profiles with in-depth information on experimental procedures along with an example of usage of these data.
动脉粥样硬化易发生于血流紊乱（d-flow）暴露的动脉区域，部分原因是内皮细胞中基因表达的改变。虽然许多体外研究表明抗动脉粥样硬化血流和促动脉粥样硬化血流如何不同地调节培养的内皮细胞的基因表达，但类似的体内研究却很少。最近，我们开发了一种动脉粥样硬化小鼠模型，通过部分结扎左侧颈动脉（LCA）分支，同时将对侧右侧颈动脉（RCA）作为对照，可快速发展出稳健的动脉粥样硬化。我们还开发了一种从这些动物颈动脉中富集内皮细胞 RNA 的新方法，这使我们能够对暴露于 d-flow 或 s-flow 的动脉内皮中的 mRNAs 和 miRNAs 进行全基因组表达分析。这些微阵列结果用于鉴定新的机械敏感性基因，如 DNA 甲基转移酶-1 和 miR-712，它们在动脉粥样硬化中发挥关键作用。在这里，我们报告了这些内皮细胞 mRNA 和 miRNA 的表达谱，以及详细的实验程序信息，并提供了这些数据的使用示例。
