EMBL/CRG Research Unit in Systems Biology, Centre for Genomic Regulation (CRG) , 08003 Barcelona, Spain ; Universitat Pompeu Fabra (UPF) , 08002 Barcelona, Spain.
Sci Data. 2015 Mar 3;2:150005. doi: 10.1038/sdata.2015.5. eCollection 2015.
Gap genes are involved in segment determination during early development in dipteran insects (flies, midges, and mosquitoes). We carried out a systematic quantitative comparative analysis of the gap gene network across different dipteran species. Our work provides mechanistic insights into the evolution of this pattern-forming network. As a central component of our project, we created a high-resolution quantitative spatio-temporal data set of gap and maternal co-ordinate gene expression in the blastoderm embryo of the non-drosophilid scuttle fly, Megaselia abdita. Our data include expression patterns in both wild-type and RNAi-treated embryos. The data-covering 10 genes, 10 time points, and over 1,000 individual embryos-consist of original embryo images, quantified expression profiles, extracted positions of expression boundaries, and integrated expression patterns, plus metadata and intermediate processing steps. These data provide a valuable resource for researchers interested in the comparative study of gene regulatory networks and pattern formation, an essential step towards a more quantitative and mechanistic understanding of developmental evolution.
间隙基因参与双翅目昆虫（蝇类、摇蚊和蚊子）早期发育中的体节确定。我们对不同双翅目物种的间隙基因网络进行了系统的定量比较分析。我们的工作为这一模式形成网络的进化提供了机制上的见解。作为我们项目的一个核心组成部分，我们创建了一个高分辨率的定量时空数据集，用于研究非果蝇类的粪蝇 Megaselia abdita 的原肠胚中的间隙基因和母体坐标基因的表达。我们的数据包括在野生型和 RNAi 处理的胚胎中的表达模式。这些数据涵盖了 10 个基因、10 个时间点和 1000 多个个体胚胎，包括原始胚胎图像、量化的表达谱、提取的表达边界位置以及整合的表达模式，加上元数据和中间处理步骤。这些数据为有兴趣研究基因调控网络和模式形成的比较研究的研究人员提供了有价值的资源，是朝着更定量和更具机制性地理解发育进化迈出的重要一步。
