活细胞中重组蛋白的光触发荧光标记
Photo-triggered fluorescent labelling of recombinant proteins in live cells.
作者信息
Jung Deokho, Sato Kohei, Min Kyoungmi, Shigenaga Akira, Jung Juyeon, Otaka Akira, Kwon Youngeun
机构信息
Department of Biomedical Engineering, Dongguk University-Seoul, Pildong 3-ga, Seoul, Korea.
出版信息
Chem Commun (Camb). 2015 Jun 14;51(47):9670-3. doi: 10.1039/c5cc01067e.
PMID:25977944
Abstract
A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.
摘要
开发了一种在活细胞中光化学触发蛋白质荧光标记的方法。该方法基于光笼式分裂内含肽介导的条件性蛋白质反式剪接反应，能够在必要的时空控制下对目标蛋白质进行无背景荧光标记。
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