BioInstrumentation Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 3-147, Cambridge, MA 02139, USA.
BioInstrumentation Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 3-147, Cambridge, MA 02139, USA.
J Control Release. 2015 Aug 10;211:37-43. doi: 10.1016/j.jconrel.2015.05.264. Epub 2015 May 13.
We present a novel method of quickly acquiring dermal interstitial fluid (ISF) samples using a Lorentz-force actuated needle-free jet injector. The feasibility of the method is first demonstrated on post-mortem porcine tissue. The jet injector is used to first inject a small volume of physiological saline to breach the skin, and the back-drivability of the actuator is utilized to create negative pressure in the ampoule and collect ISF. The effect of the injection and extraction parameters on sample dilution and extracted volumes is investigated. A simple finite element model is developed to demonstrate why this acquisition method results in faster extractions than conventional sampling methods. Using this method, we are able to collect a sample that contains up to 3.5% ISF in 3.1s from post-mortem skin. The trends revealed from experimentation on post-mortem skin are then used to identify the parameters for a live animal study. The feasibility of the acquisition process is successfully demonstrated using live rats; the process is revealed to extract samples that have been diluted by a factor of 111-125.
我们提出了一种使用洛伦兹力驱动的无针喷射注射器快速获取真皮间质液 (ISF) 样本的新方法。该方法的可行性首先在死后猪组织上进行了验证。该喷射注射器首先用于注射小体积的生理盐水以刺破皮肤，并且利用致动器的反驱动力在安瓿中产生负压并收集 ISF。研究了注射和提取参数对样品稀释和提取量的影响。开发了一个简单的有限元模型来演示为什么这种采集方法比传统的采样方法导致更快的提取。使用这种方法，我们能够在 3.1 秒内从死后皮肤中收集含有高达 3.5% ISF 的样品。然后，使用死后皮肤上的实验结果来确定活体动物研究的参数。使用活体大鼠成功地证明了采集过程的可行性；该过程被证明可以提取已稀释 111-125 倍的样品。
