Chen Ningqing, Zheng Siyu
Zhongguo Yi Liao Qi Xie Za Zhi. 2014 Nov;38(6):458-60.
To investigate the biuret reagent to detect proteins in the application, the impact of different test conditions for test results.
The biuret method to select three different instruments, reagents, calibrators are arranged in combination to form 27 sets of detection systems, each detection system is a combination of 5 serum samples for testing, 5 measured values obtained, the selection process normality good a serum for the study to determine the mean value of all AST after culling outliers obtained in order to calculate the various detection systems use a combination of biuret reagent to detect proteins bias.
The use of different detection equipment to detect proteins biuret reagent bias, homogeneity of variance (P = 0.467), the difference was not statistically significant (F = 1.688, P = 0.421). different detection reagents using biuret reagent to detect proteins bias, homogeneity of variance (P = 0.574), a statistically significant difference (F = 5.784, P = 0.011). different calibrators use biuret reagent to detect proteins bias, homogeneity of variance (P = 0.467), the difference was statistically significant (F = 5.289, P = 0.000).
Biuret reagent in the detection of protein applications, impact detection reagents and calibrators will test result, during the test than when it is necessary to detect deviation detection reagents and calibrators due to be considered.
探讨双缩脲试剂在检测蛋白质中的应用，不同检测条件对检测结果的影响。
采用双缩脲法，选择三种不同仪器、试剂、校准品进行组合，形成27套检测系统，每套检测系统用5份血清样本进行检测，得到5个测量值，选择过程正常性良好的一份血清用于研究，剔除异常值后确定所有天冬氨酸转氨酶的均值，以此计算各检测系统使用双缩脲试剂检测蛋白质的偏倚。
使用不同检测设备用双缩脲试剂检测蛋白质的偏倚，方差齐性（P = 0.467），差异无统计学意义（F = 1.688，P = 0.421）。使用不同检测试剂用双缩脲试剂检测蛋白质的偏倚，方差齐性（P = 0.574），差异有统计学意义（F = 5.784，P = 0.011）。使用不同校准品用双缩脲试剂检测蛋白质的偏倚，方差齐性（P = 0.467），差异有统计学意义（F = 5.289，P = 0.000）。
双缩脲试剂在检测蛋白质应用中，检测试剂和校准品会影响检测结果，检测时比有必要考虑检测试剂和校准品引起的检测偏差。
