Shideler C E, Stewart K K, Crump J, Wills M R, Savory J, Renoe B W
Clin Chem. 1980 Sep;26(10):1454-8.
We have examined the feasibility of the automated multiple flow-injection technique for application to clinical chemistry by adapting to this system the biuret method for the determination of total protein. Samples were discretely and rapidly introduced into a continuously flowing, nonsegmented reagent stream by means of an automatic sampler and high-pressure injection valve. Pumps operating at 1380-2070 kPa (200-300 psi) were utilized to introduce the biuret reagent and saline diluent into the system separately at flow rates of 72 and 47 microL/s, respectively. Use of 20-microL sample and a 3.0-s reaction-delay coil was adequately sensitive for analysis for total protein by this method. Samples were analyzed at a rate of 150/h with no detectable between-sample carryover. Within-run precision studies yielded relative standard deviations of 2.5% and less. Total protein values obtained by this method correlated well with those obtained by centrifugal analyzer and bubble-segmented continuous-flow biuret methods.
我们通过将双缩脲法用于总蛋白测定并应用于该系统，研究了自动多重流动注射技术应用于临床化学的可行性。样品通过自动进样器和高压进样阀被离散且快速地引入到连续流动、无分段的试剂流中。使用工作压力为1380 - 2070 kPa（200 - 300 psi）的泵，分别以72和47 μL/s的流速将双缩脲试剂和生理盐水稀释剂引入系统。使用20 μL样品和3.0 s的反应延迟盘管，该方法对总蛋白分析具有足够的灵敏度。样品以150个/小时的速率进行分析，未检测到样品间的残留。批内精密度研究的相对标准偏差为2.5%及以下。通过该方法获得的总蛋白值与通过离心分析仪和气泡分段连续流动双缩脲法获得的值具有良好的相关性。
