Wang Min, Song Yongli, Wang Jimeng, Yan Hui, Mi Wenjuan, Qiu Jianhua, Qiao Li
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Dec;28(23):1884-7.
This study was designed to find out the impact of micro-ecological environment on the incidence of allergic rhinitis after developing a model of allergic rhinitis on mice.
Sixty mice were randomly divided into GF group (n=30) and SPF group (n=30). Mice of GF group were fed in the germ-free environment and mice of SPF group were fed in the specific pathogen-free environment. Then each group were randomly divided into model group (20 mice) and control group (10 mice). Establish allergic rhinitis model in the mice of model group using ovalbumin (OVA) at the age of 6 weeks, observe and score the corresponding symptoms and signs that could been induced. Stain with hematoxylin eosin (HE) staining method for nasal mucosa to observe the morphological changes. Using enzyme linked immunosorbent assay to detect the concentration of IgE, IFN-γ and IL-4 in the peripheral blood serum.
The chi square test showed that the incidence of allergic rhinithis in the mice of GF group was significantly higher than that in the SPF group (P< 0. 05). HE staining showed that the nasal mucosas of allergic rhinitis positive reaction mice were highly congestive and edematous and had a large number of inflammatory cell infiltration, while there was no abnormal morphology of nasal mucosas in mice with no allergic rhinitis reaction. EOS counting displayed that the number of eosinophilic cells in nasal mucosa of positive allergic rhinitis reaction mice was increased significantly. The concentration of IgE and IL-4 in the serum of positive allergic rhinitis reaction mice was highly increased (P <0. 05), and IFN-γ was significantly decreased (P< 0.05).
The difference of micro-ecological environment may play a key role in the occurrence of allergic rhinitis in mice.
本研究旨在通过建立小鼠变应性鼻炎模型，探究微生态环境对变应性鼻炎发病率的影响。
将60只小鼠随机分为无菌组（n = 30）和无特定病原体组（SPF组，n = 30）。无菌组小鼠在无菌环境中饲养，SPF组小鼠在无特定病原体环境中饲养。然后每组再随机分为模型组（20只小鼠）和对照组（10只小鼠）。6周龄时，用卵清蛋白（OVA）对模型组小鼠建立变应性鼻炎模型，观察并记录诱导产生的相应症状和体征并进行评分。采用苏木精-伊红（HE）染色法对鼻黏膜进行染色，观察形态学变化。用酶联免疫吸附测定法检测外周血血清中IgE、IFN-γ和IL-4的浓度。
卡方检验显示，无菌组小鼠变应性鼻炎的发病率显著高于SPF组（P < 0.05）。HE染色显示，变应性鼻炎阳性反应小鼠的鼻黏膜高度充血、水肿，有大量炎性细胞浸润，而无变应性鼻炎反应小鼠的鼻黏膜形态无异常。嗜酸性粒细胞计数显示，变应性鼻炎阳性反应小鼠鼻黏膜中嗜酸性粒细胞数量显著增加。变应性鼻炎阳性反应小鼠血清中IgE和IL-4的浓度显著升高（P < 0.05），而IFN-γ显著降低（P < 0.05）。
微生态环境的差异可能在小鼠变应性鼻炎的发生中起关键作用。
