Department of Otolaryngology-Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, China.
Department of Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, China.
J Infect Public Health. 2021 Jan;14(1):77-83. doi: 10.1016/j.jiph.2019.09.010. Epub 2019 Nov 18.
The objective of this study is to analyze the expression of ILC2 cells (type 2 innate lymphoid cells) in nasal mucosa based on animal model of allergic bacterial infection rhinitis. 45 female BALB/c mice were selected as research subject. They were randomly divided into control group (group A), sensitization group (group B) and inhibitor group (group C) to establish a mouse model of allergic rhinitis. The pathological changes of mouse nasal mucosa were observed by HE (hematoxylin-eosin) staining. The number of ILC2 cells in mouse nasal mucosa was detected by immunofluorescence double staining assay. Real-time quantitative Polymerase Chain Reaction (PCR) was used to detect the expression of ILC2 cell-associated factor in mouse nasal mucosa. The expression of cytokine protein in serum was detected by enzyme linked immunosorbent assay. The results showed that there was no inflammatory cell infiltration in the nasal mucosa of group A, and the number of ILC2 cells was small. Inflammatory cell infiltration and obvious ILC2 cells were observed in the nasal mucosa of group B and C, and the number of ILC2 cells in group B and C was significantly increased compared with that in group A. Compared with group A, ROR α, Thy-1, ST2, and CD90 genes were significantly increased in nasal mucosa tissues of group B and C, and protein levels of IL-4, IL-5, IL-13, and IgE in serum were significantly increased. Compared with group B, the protein expression levels of IL-13 and IgE in serum of group C mice were significantly increased, while the expression levels of IL-4 and IL-5 were not significantly different. In conclusion, in the pathogenesis of allergic rhinitis, ILC2 cells play a role in promoting the development of inflammation, and its expression is related to RORα, Thy-1, ST2 and CD90. Meanwhile, ILC2 cells are also important cells for the synthesis and secretion of IL-13. The study on the pathogenesis of allergic rhinitis provides a new target for its treatment.
本研究旨在基于变应性细菌性感染性鼻炎动物模型，分析鼻黏膜中 ILC2 细胞（2 型固有淋巴细胞）的表达。选择 45 只雌性 BALB/c 小鼠作为研究对象，随机分为对照组（A 组）、致敏组（B 组）和抑制剂组（C 组），建立变应性鼻炎小鼠模型。采用 HE（苏木精-伊红）染色观察各组小鼠鼻黏膜的病理变化，免疫荧光双染法检测各组小鼠鼻黏膜中 ILC2 细胞的数量，实时定量 PCR 检测各组小鼠鼻黏膜中 ILC2 细胞相关因子的表达，酶联免疫吸附试验检测各组小鼠血清中细胞因子蛋白的表达。结果显示，A 组鼻黏膜无炎性细胞浸润，ILC2 细胞数量较少；B、C 组鼻黏膜可见炎性细胞浸润及明显的 ILC2 细胞，B、C 组 ILC2 细胞数量明显高于 A 组。与 A 组相比，B、C 组鼻黏膜组织 RORα、Thy-1、ST2、CD90 基因表达显著增加，血清中 IL-4、IL-5、IL-13、IgE 蛋白水平显著升高；与 B 组相比，C 组小鼠血清中 IL-13、IgE 蛋白表达水平显著升高，IL-4、IL-5 蛋白表达水平无显著差异。结论：在变应性鼻炎发病机制中，ILC2 细胞在促进炎症发展中发挥作用，其表达与 RORα、Thy-1、ST2、CD90 有关；同时，ILC2 细胞也是 IL-13 合成和分泌的重要细胞。本研究为其治疗提供了新的靶点。
