Shoibonov B B, Kravchenko M A, Baronets V Y, Tolpygo S M, Kostyreva M V, Shabalina A A, Zamolodchikova T S, Kotov A V, Panchenko L F
Patol Fiziol Eksp Ter. 2014 Oct-Dec(4):133-8.
A method for determining atherogenicity of the immune complexes containing multiple modified low-density lipoprotein (mmLDL) in complement fixation test has been found. In the proposed method, the precipitate immune complexes containing mmLDL (IC mmLDL) was prepared from human serum by treating it with buffer (8.3% of th PEG 3350 and 3.3% PVP 12600 th in the ratio 1: 1.2) for 10 min at 23 °C. IC mmLDL aggregates were separated by centrifugation at 3100g for 10 min at 23 °C. The precipitate IC mmLDL was dissolved in buffer without PEG and PVP, cholesterol content and the degree of binding of guinea pig complement were measured. Atherogenicity of the IC mmLDL was registered as the ratio of the degree of complement binding to cholesterol in the the immune complexes.
已经发现一种在补体结合试验中测定含有多种修饰低密度脂蛋白（mmLDL）的免疫复合物致动脉粥样硬化性的方法。在所提出的方法中，通过在23℃下用缓冲液（8.3%的PEG 3350和3.3%的PVP 12600，比例为1:1.2）处理人血清10分钟，制备含有mmLDL的沉淀免疫复合物（IC mmLDL）。在23℃下以3100g离心10分钟分离IC mmLDL聚集体。将沉淀的IC mmLDL溶解在不含PEG和PVP的缓冲液中，测量胆固醇含量和豚鼠补体的结合程度。IC mmLDL的致动脉粥样硬化性记为免疫复合物中补体结合程度与胆固醇的比率。
