Shoibonov B B, Baronets V Yu, Panchenko L F, Kubatiev A A
Patol Fiziol Eksp Ter. 2013 Oct-Dec(4):109-14.
A method of rapid determination of cholesterol in immune complexes (CIC). In the proposed method precipitate immune complexes containing multiple modified low density lipoproteins from human serum are prepared by treatment with a buffer containing 8.3 PEC 3350, and 3.3% PVP 12600, in a ratio of 1:1.2, incubated for 10 min at room temperature. The precipitate containing the CIC is separated by centrifugation at 3100 g for 5 min at 23 degrees C, dissolved in a buffer without PEG and PVP, the cholesterol is determined using an enzymatic kit and at a level of CIC more than 8.3 mg/dl ascertain higher level. The method improves the accuracy of the quantitative determination of CIC, conduct extensive screening tests to detect atherosclerosis as the pre-clinical stage and monitor the effectiveness of the therapy.
一种快速测定免疫复合物（CIC）中胆固醇的方法。在所提出的方法中，通过用含有8.3%聚乙二醇3350（PEC 3350）和3.3%聚乙烯吡咯烷酮12600（PVP 12600）的缓冲液按1:1.2的比例处理人血清，制备含有多种修饰低密度脂蛋白的沉淀免疫复合物，在室温下孵育10分钟。通过在23℃下以3100g离心5分钟分离含有CIC的沉淀，将其溶解在不含聚乙二醇和聚乙烯吡咯烷酮的缓冲液中，使用酶试剂盒测定胆固醇，当CIC水平超过8.3mg/dl时确定为更高水平。该方法提高了CIC定量测定的准确性，可进行广泛的筛查试验以检测动脉粥样硬化的临床前期阶段并监测治疗效果。
