使用光场重建方法进行细胞观察的相衬显微镜术。

Phase microscopy using light-field reconstruction method for cell observation.

作者信息

Xiu Peng, Zhou Xin, Kuang Cuifang, Xu Yingke, Liu Xu

机构信息

State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, China.

出版信息

Micron. 2015 Aug;75:11-7. doi: 10.1016/j.micron.2015.04.009. Epub 2015 Apr 27.

DOI:10.1016/j.micron.2015.04.009

PMID:25980387

Abstract

The refractive index (RI) distribution can serve as a natural label for undyed cell imaging. However, the majority of images obtained through quantitative phase microscopy is integrated along the illumination angle and cannot reflect additional information about the refractive map on a certain plane. Herein, a light-field reconstruction method to image the RI map within a depth of 0.2 μm is proposed. It records quantitative phase-delay images using a four-step phase shifting method in different directions and then reconstructs a similar scattered light field for the refractive sample on the focus plane. It can image the RI of samples, transparent cell samples in particular, in a manner similar to the observation of scattering characteristics. The light-field reconstruction method is therefore a powerful tool for use in cytobiology studies.

摘要

折射率（RI）分布可作为未染色细胞成像的天然标记。然而，通过定量相显微镜获得的大多数图像是沿照明角度积分的，无法反映特定平面上折射图的额外信息。在此，提出了一种用于对0.2μm深度范围内的RI图进行成像的光场重建方法。它使用四步相移法在不同方向记录定量相位延迟图像，然后为焦平面上的折射样品重建类似的散射光场。它能够以类似于观察散射特性的方式对样品，特别是透明细胞样品的RI进行成像。因此，光场重建方法是细胞生物学研究中的一种强大工具。