利用高分辨率熔解分析快速高效地区分耶尔森菌属菌种。
Rapid and efficient differentiation of Yersinia species using high-resolution melting analysis.
作者信息
Souza Roberto A, Frazão Miliane R, Almeida Alzira M P, Falcão Juliana P
机构信息
Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil.
Brazilian Reference Service on Plague, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (CPqAM/FIOCRUZ), Recife, PE, Brazil.
出版信息
J Microbiol Methods. 2015 Aug;115:6-12. doi: 10.1016/j.mimet.2015.05.014. Epub 2015 May 14.
The primary goal of clinical microbiology is the accurate identification of the causative agent of the disease. Here, we describe a method for differentiation between Yersinia species using PCR-HRMA. The results revealed species-specific melting profiles. The herein developed assay can be used as an effective method to differentiate Yersinia species.
临床微生物学的主要目标是准确鉴定疾病的病原体。在此，我们描述了一种使用PCR-HRMA区分耶尔森菌属菌种的方法。结果显示了菌种特异性的熔解曲线。本文开发的检测方法可作为区分耶尔森菌属菌种的有效方法。
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