Zhang Yanan, Wang Zhanli, Wang Wenrui, Yu Hui, Jin Min
Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou, Inner Mongolia 014060, P.R. China.
General Center for Disease Control and Prevention of Inner Mongolia Autonomous Region, Huhehot, Inner Mongolia 010031, P.R. China.
Exp Ther Med. 2022 Jun 14;24(2):511. doi: 10.3892/etm.2022.11438. eCollection 2022 Aug.
Plague is an acute bacterial infection caused by . The three major clinical forms of plague are bubonic, pneumonic and septicemic, which have high case-mortality rates. Therefore, rapid and reliable diagnostic tools are crucial. Currently, bacteriological means and traditional serological assays are used for detecting infection with . However, such methods have their limitations. Polymerase chain reaction (PCR) is one of the most useful tools for rapid diagnosis of plague. The present review introduced the main PCR techniques and their applications for detecting and confirmation of . The advantages and disadvantages of the different PCR methods were also summarized.
鼠疫是由……引起的一种急性细菌感染。鼠疫的三种主要临床类型为腺鼠疫、肺鼠疫和败血症型鼠疫，病死率很高。因此，快速可靠的诊断工具至关重要。目前，细菌学方法和传统血清学检测用于检测……感染。然而，这些方法有其局限性。聚合酶链反应（PCR）是快速诊断鼠疫最有用的工具之一。本综述介绍了主要的PCR技术及其在检测和确认……方面的应用。还总结了不同PCR方法的优缺点。
