He Qun, Wang Xinghao, Sun Ping, Wang Zunyao, Wang Liansheng
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
Aquat Toxicol. 2015 Jul;164:145-54. doi: 10.1016/j.aquatox.2015.05.005. Epub 2015 May 6.
Tetrabromobisphenol A (TBBPA) is a well-known brominated flame retardant. It has been detected in the environment and shows high acute toxicity to different organisms at high concentrations. In this work, the effects of pH and dimethyl sulfoxide (DMSO) on the acute toxicity of TBBPA to Daphnia magna and Limnodrilus hoffmeisteri were tested, and the oxidative stress induced by TBBPA in livers of Carassius auratus was assessed using four biomarkers. The integrated biomarker response (IBR) was applied to assess the overall antioxidant status in fish livers. Moreover, fish tissues (gills and livers) were also studied histologically. The results showed that low pH and DMSO enhanced the toxicity of TBBPA. Furthermore, changes in the activity of antioxidant enzymes and glutathione level suggested that TBBPA generates oxidative stress in fish livers. The IBR index revealed that fish exposed to 3mg/L TBBPA experienced more serious oxidative stress than exposed to acidic or alkaline conditions. The histopathological analysis revealed lesions caused by TBBPA. This study provides valuable toxicological information of TBBPA and will facilitate a deeper understanding on its potential toxicity in realistic aquatic environments.
四溴双酚A（TBBPA）是一种著名的溴化阻燃剂。它已在环境中被检测到，并且在高浓度时对不同生物表现出高急性毒性。在这项工作中，测试了pH值和二甲基亚砜（DMSO）对TBBPA对大型溞和霍甫水丝蚓急性毒性的影响，并使用四种生物标志物评估了TBBPA在鲫鱼肝脏中诱导的氧化应激。应用综合生物标志物反应（IBR）来评估鱼肝中的整体抗氧化状态。此外，还对鱼组织（鳃和肝脏）进行了组织学研究。结果表明，低pH值和DMSO增强了TBBPA的毒性。此外，抗氧化酶活性和谷胱甘肽水平的变化表明TBBPA在鱼肝中产生氧化应激。IBR指数显示，暴露于3mg/L TBBPA的鱼比暴露于酸性或碱性条件下经历更严重的氧化应激。组织病理学分析揭示了由TBBPA引起的病变。本研究提供了TBBPA有价值的毒理学信息，并将有助于更深入了解其在实际水生环境中的潜在毒性。
