Yamada Kentaro, Narimatsu Yuri, Ono Yumie, Sasaguri Ken-Ichi, Onozuka Minoru, Kawata Toshitsugu, Yamamoto Toshiharu
Department of Oral Science, Division of Brain Functions and Neuroscience, Kanagawa Dental University, Yokosuka, Japan.
Department of Oral Science, Division of Orthodontics, Kanagawa Dental University, Yokosuka, Japan.
Neurosci Lett. 2015 Jul 10;599:43-8. doi: 10.1016/j.neulet.2015.05.023. Epub 2015 May 14.
We investigated the effects of chewing under immobilization stress on the periaqueductal gray (PAG) matter using phosphorylated extracellular signal-regulated kinase (pERK) as a marker of responding cells. Immobilization stress increased pERK-immunoreactive cells in the PAG. Among four subdivisions of the PAG, the increase of immunoreactive cells was remarkable in the dorsolateral and ventrolateral subdivisions. However, increase of pERK-immunoreactive cells by the immobilization stress was not so evident in the dorsomedial and lateral subdivisions. The chewing under immobilization stress prevented the stress-induced increase of pERK-immunoreactive cells in the dorsolateral and ventrolateral subdivisions with statistical significances (p<0.05). Again, chewing effects on pERK-immunoreactive cells were not visible in the dorsomedial and lateral subdivisions. These results suggest that the chewing alleviates the PAG (dorsolateral and ventrolateral subdivisions) responses to stress.
我们以磷酸化细胞外信号调节激酶（pERK）作为反应细胞的标志物，研究了固定应激下咀嚼对中脑导水管周围灰质（PAG）的影响。固定应激增加了PAG中pERK免疫反应性细胞的数量。在PAG的四个亚区中，背外侧和腹外侧亚区的免疫反应性细胞增加显著。然而，固定应激引起的pERK免疫反应性细胞增加在背内侧和外侧亚区并不明显。固定应激下的咀嚼可防止背外侧和腹外侧亚区应激诱导的pERK免疫反应性细胞增加，具有统计学意义（p<0.05）。同样，咀嚼对背内侧和外侧亚区pERK免疫反应性细胞的影响不明显。这些结果表明，咀嚼可减轻PAG（背外侧和腹外侧亚区）对应激的反应。
