Orthodontic Division, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, Inaoka-Cho 82, Yokosuka, Kanagawa, 238-8580, Japan.
Department of Oral Surgery, Jichi Medical University Hospital, Yakushiji, 3311-1, Shimotsuke, Tochigi, 329-0498, Japan.
Neurosci Lett. 2020 May 14;727:134921. doi: 10.1016/j.neulet.2020.134921. Epub 2020 Mar 19.
The eff ;ects of chewing during restraint stress on the anterior, mid- and posterior cingulate cortices were investigated in rats using immunohistochemistry to detect the expression of phosphorylated extracellular signal-regulated kinase 1 and 2 (pERK1/2), a marker of responding cells. The rats were divided into three groups: control (no immobilization), stress-only (immobilized), and stress-with-chewing (immobilized and allowed to chew a wooden stick). Significant increases in the number of pERK1/2-immunoreactive cells in the anterior, mid- and posterior cingulate cortices were noted in the stress-only group when compared with the control group (p < 0.05). Furthermore, the number of pERK1/2-immunoreactive cells in the anterior, mid- and posterior cingulate cortices in the stress-with-chewing group was also significantly higher than that in the stress-only group (p < 0.05). These findings indicate that the cingulate cortex plays a role in the negative-feedback effect and might be an essential part of the brain where the ameliorating effects of chewing against stress are produced.
使用免疫组织化学方法检测磷酸化细胞外信号调节激酶 1 和 2（pERK1/2）的表达，来研究束缚应激时咀嚼对大鼠前扣带回皮质、中扣带回皮质和后扣带回皮质的影响，pERK1/2 是反应细胞的标志物。将大鼠分为三组：对照组（无固定）、应激组（固定）和应激加咀嚼组（固定并允许咀嚼木棍）。与对照组相比，应激组大鼠前扣带回皮质、中扣带回皮质和后扣带回皮质中 pERK1/2 免疫反应细胞的数量明显增加（p<0.05）。此外，应激加咀嚼组大鼠前扣带回皮质、中扣带回皮质和后扣带回皮质中 pERK1/2 免疫反应细胞的数量也明显高于应激组（p<0.05）。这些发现表明扣带回皮质在负反馈效应中起作用，可能是咀嚼对抗应激的缓解作用产生的大脑的重要组成部分。
