虎杖苷与白藜芦醇在体内相互转化及抗氧化作用的比较研究。
Comparative studies of polydatin and resveratrol on mutual transformation and antioxidative effect in vivo.
机构信息
Department of Pharmacology, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200 Cailun Road, Shanghai 201203, China.
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
出版信息
Phytomedicine. 2015 May 15;22(5):553-9. doi: 10.1016/j.phymed.2015.03.014. Epub 2015 Apr 6.
BACKGROUND
Polydatin and resveratrol are extractives of radix or rhizoma of Polygonum cuspidatum, and as the glycoside forms, it is a natural precursor of resveratrol.
PURPOSE
In this study, we aimed to explore the mutual transformation between polydatin and resveratrol in rats, and to compare the antioxidative effect of them in vivo.
STUDY DESIGN
In this study, we analyzed the serum molar concentration of polydatin and resveratrol after oral administration in rats and evaluated the anti-oxidative stress effects of them using a mouse model.
METHODS
Rats were orally administered polydatin or resveratrol and the concentration of them in serum were analyzed by high performance liquid chromatography (HPLC). Their antioxidative effect was compared in mice with oxidative stress cardiomyopathy induced by doxorubicin (DOX).
RESULTS
The results showed that polydatin and resveratrol could mutually transform in vivo, the molar concentration of polydatin in serum was always averagely 3.35 and 4.28 times as much as resveratrol after oral administration of polydatin and resveratrol at 200 mg/kg, respectively. Both polydatin and resveratrol could significantly decrease the content of malonydialdehyde (MDA), promote the activities of total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in plasma, and increase the content of glutathione (GSH) in myocardial tissue. The effect of polydatin surpassed resveratrol, particularly embodied in increasing the activities of T-SOD and CAT, and the content of GSH.
CONCLUSION
It illustrates that polydatin is the main substance in serum after intragastric administration with polydatin or resveratrol, and the mutual transformation between polydatin and resveratrol keeps balance; they both have the ability of antioxidative stress in vivo, and polydatin has a better effect than resveratrol, which hints that polydatin may be a substitute for resveratrol in antioxidant for clinical use.
背景
白藜芦醇苷和白藜芦醇是虎杖的根茎提取物，且以糖苷的形式存在，是白藜芦醇的天然前体。
目的
本研究旨在探讨白藜芦醇苷和白藜芦醇在大鼠体内的相互转化，并比较它们的体内抗氧化作用。
设计
本研究通过高效液相色谱法（HPLC）分析大鼠口服白藜芦醇苷和白藜芦醇后血清中两者的摩尔浓度，并采用阿霉素（DOX）诱导的氧化应激性心肌病小鼠模型评价它们的抗氧化应激作用。
方法
大鼠灌胃白藜芦醇苷或白藜芦醇，采用 HPLC 法分析血清中药物浓度。采用 DOX 诱导氧化应激性心肌病小鼠模型比较白藜芦醇苷和白藜芦醇的抗氧化作用。
结果
结果表明，白藜芦醇苷和白藜芦醇在体内可相互转化，大鼠灌胃 200mg/kg 白藜芦醇苷和白藜芦醇后，血清中白藜芦醇苷摩尔浓度分别为白藜芦醇的 3.35 倍和 4.28 倍。白藜芦醇苷和白藜芦醇均可显著降低丙二醛（MDA）含量，提高血浆中超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性，增加心肌组织中谷胱甘肽（GSH）含量。白藜芦醇苷作用强于白藜芦醇，尤其体现在提高 T-SOD 和 CAT 活性及 GSH 含量方面。
结论
提示灌胃白藜芦醇苷或白藜芦醇后，血清中主要物质为白藜芦醇苷，且白藜芦醇苷和白藜芦醇相互转化处于平衡状态；两者均具有体内抗氧化应激作用，白藜芦醇苷作用优于白藜芦醇，提示白藜芦醇苷可能成为临床抗氧化的白藜芦醇替代药物。
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