Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, People's Republic of China.
Life Sci. 2012 Oct 29;91(17-18):872-7. doi: 10.1016/j.lfs.2012.08.033. Epub 2012 Sep 12.
Resveratrol (Res) which is a polyphenolic phytoalexin, has various biological properties. In the present study, we investigated whether Res extracted from Polygonum cuspidatum can reduce oxidative damage and cognitive impairment in senescence-accelerated mouse (SAM).
Senescence-accelerated mice were administered with Res (25, 50, 100mg·kg(-1)·d(-1)) for 8weeks. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in mice brain were determined. The gene expression of SOD in mice brain was investigated by real time reverse transcriptase-polymerase chain reaction (RT-PCR).
The results showed that resveratrol significantly improved learning and memory ability in Morris water maze test and neuromuscular coordination and sensorimotor capacity in tightrope test. Meanwhile, Res increased the activities of antioxidant enzymes with a reduction in lipid peroxidation. And real time RT-PCR analysis also indicated that the change of SOD mRNA was the same as the modification of SOD activity in mice brain. Furthermore, Res could prevent cerebral mitochondrial DNA deletions which might be one of the causes resulting in learning and memory impairment.
These results suggest that the pharmacological action of Res may offer a novel therapeutic strategy for the treatment of age-related conditions.
白藜芦醇（Res）是一种多酚类植物抗毒素，具有多种生物学特性。在本研究中，我们研究了是否能从虎杖中提取的白藜芦醇来减轻加速衰老小鼠（SAM）的氧化损伤和认知障碍。
用白藜芦醇（25、50、100mg·kg(-1)·d(-1)）处理加速衰老小鼠 8 周。测定小鼠大脑中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性和丙二醛（MDA）的含量。通过实时逆转录聚合酶链反应（RT-PCR）研究小鼠大脑中 SOD 的基因表达。
结果表明，白藜芦醇能显著改善 Morris 水迷宫试验中的学习和记忆能力，以及绳梯试验中的神经肌肉协调性和感觉运动能力。同时，Res 增加了抗氧化酶的活性，降低了脂质过氧化。实时 RT-PCR 分析也表明，SOD mRNA 的变化与小鼠大脑中 SOD 活性的修饰一致。此外，Res 可以预防大脑线粒体 DNA 缺失，这可能是导致学习和记忆障碍的原因之一。
这些结果表明，Res 的药理作用可能为治疗与年龄相关的疾病提供一种新的治疗策略。
