Zigová T, Marsala J, Monti Graziadei G, Graziadei P P
Cesk Neurol Neurochir. 1989 Sep;52(5):306-12.
Embryonic tissue (E14-E16) of the olfactory bulb and substantia nigra resp. was transplanted to neonatal rats into cavities formed by partial or total unilateral bulbectomy. After 40 days of survival the animals were perfused with Bouin's fluid and processed for histological examination. Complete series of 10 microns horizontal or sagittal sections were stained with Gill haematoxylin and Bodian's silver method. To demonstrate the olfactory protein immunohistochemical staining with peroxidase-antiperoxidase was used. After bulbectomy degeneration of the axons of the olfactory cells occur, which subsequently regenerate, penetrate into the transplant and form typical glomerular structures in the latter. By immunohistochemical staining in both types of transplants olfactory structures were reliably differentiated from non-olfactory elements.
分别将嗅球和黑质的胚胎组织（胚胎期14 - 16天）移植到新生大鼠体内，移植部位是通过部分或完全单侧嗅球切除形成的腔隙。存活40天后，用布因氏液灌注动物，并进行组织学检查。制作10微米厚的完整水平或矢状切片系列，用吉尔苏木精和博迪安银法染色。为了显示嗅觉蛋白，采用过氧化物酶 - 抗过氧化物酶免疫组织化学染色法。嗅球切除后，嗅细胞轴突发生退变，随后再生，穿透进入移植组织，并在其中形成典型的小球状结构。通过免疫组织化学染色，在两种类型的移植组织中，嗅觉结构都能与非嗅觉成分可靠地区分开来。
