Amemori T, Ermakova I V, Buresová O, Zigová T, Racekova E, Bures J
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Behav Neurosci. 1989 Feb;103(1):61-70. doi: 10.1037//0735-7044.103.1.61.
The possibility to compensate the loss of olfactory and non-olfactory functions due to removal of the olfactory bulb by embryonal brain grafts was investigated in adult rats. Spatial working memory was examined in an 8-arm radial water maze task 6 weeks after bulbectomy. During 15 daily trials, performance gradually improved in bulbectomized controls (n = 10) and in rats with olfactory bulb transplants (n = 9), but did not attain that of intact controls (n = 10). No improvement was observed in the rats with substantia nigra grafts (n = 8). Eleven weeks after bulbectomy, the same rats were tested in the water tank navigation task. The performance improved during ten 12-trial sessions in bulbectomized rats less than in intact controls, but more than in the transplanted rats. The olfactory food retrieval test performed 14 weeks after bulbectomy revealed almost full recovery of smell in bulbectomized rats, but not in the transplanted animals. It is concluded that the spatial memory deficit is probably due to bulbectomy-induced interference with septohippocampal function which is not alleviated, but rather enhanced by transplantation. The results suggest that the effect of brain grafting is not always beneficial.
在成年大鼠中研究了通过胚胎脑移植来补偿因嗅球切除而导致的嗅觉和非嗅觉功能丧失的可能性。在切除嗅球6周后，在八臂放射状水迷宫任务中检测空间工作记忆。在15天的每日试验期间，嗅球切除的对照组（n = 10）和嗅球移植的大鼠（n = 9）的表现逐渐改善，但未达到完整对照组（n = 10）的水平。在黑质移植的大鼠（n = 8）中未观察到改善。嗅球切除11周后，对相同的大鼠进行水箱导航任务测试。在十次12次试验过程中，嗅球切除的大鼠的表现有所改善，但不如完整对照组，不过比移植大鼠要好。嗅球切除14周后进行的嗅觉食物取回测试显示，嗅球切除的大鼠嗅觉几乎完全恢复，但移植动物未恢复。得出的结论是，空间记忆缺陷可能是由于嗅球切除引起的对隔海马功能的干扰，这种干扰并未因移植而减轻，反而增强了。结果表明，脑移植的效果并非总是有益的。
