Ikegawa S, Kinoshita J, Takeshita K, Sato H, Tohma M
Chem Pharm Bull (Tokyo). 1989 Aug;37(8):2147-52. doi: 10.1248/cpb.37.2147.
Anti glyco-3 beta,12 alpha-dihydroxy-5-cholen-24-oic acid antisera were prepared by immunizing rabbits with hapten-bovine serum albumin (BSA) conjugates coupled at the C-3, C-15 alpha, and C-24 positions on the bile acid molecule, and their properties were investigated by heterologous combination assay using 125I-labeled tracer. The antiserum raised against the C-3 BSA conjugate showed poor titer and specificity, while the antisera from the other two conjugates showed satisfactorily high affinity constants (Ka = 5.0 x 10(8) and 7.0 x 10(8) M-1) and reasonable specificity, exhibiting negligible cross-reactivities with other major human bile acids and cholesterol. Among the unsaturated bile acids tested, high reactivity was observed with tauro-3 beta,12 alpha-dihydroxy-5-cholen-24-oic acid, which suggested that bridge phenomena were significant in this assay system.
通过用在胆汁酸分子的C-3、C-15α和C-24位置偶联的半抗原-牛血清白蛋白（BSA）缀合物免疫兔子来制备抗糖基-3β，12α-二羟基-5-胆烷-24-酸抗血清，并使用125I标记的示踪剂通过异源结合测定来研究它们的性质。针对C-3 BSA缀合物产生的抗血清显示出较低的效价和特异性，而来自其他两种缀合物的抗血清显示出令人满意的高亲和常数（Ka = 5.0 x 10(8) 和7.0 x 10(8) M-1）和合理的特异性，与其他主要的人胆汁酸和胆固醇的交叉反应可忽略不计。在所测试的不饱和胆汁酸中，观察到与牛磺-3β，12α-二羟基-5-胆烷-24-酸有高反应性，这表明在该测定系统中桥连现象很显著。
