沙特冠状动脉疾病患者肾素-血管紧张素系统基因多态性
Renin-angiotensin system gene polymorphisms among Saudi patients with coronary artery disease.
作者信息
Al-Hazzani Amal, Daoud Mohamed S, Ataya Farid S, Fouad Dalia, Al-Jafari Abdulaziz A
机构信息
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh, 11459 Saudi Arabia.
Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451 Saudi Arabia ; King Fahd Unit Laboratory, Department of Clinical and Chemical Pathology, Kasr Al-Ainy University Hospital, Cairo University, El-Manial, Cairo, 11562 Egypt.
出版信息
J Biol Res (Thessalon). 2014 May 21;21(1):8. doi: 10.1186/2241-5793-21-8. eCollection 2014 Dec.
BACKGROUND
The polymorphisms in the components of the renin-angiotensin system (RAS) are important in the development and progression of coronary artery disease (CAD) in some individuals. Our objectives in the present investigation were to determine whether three RAS polymorphisms, angiotensin-converting enzyme insertion/deletion (ACE I/D), angiotensin receptor II (Ang II AT2 - C3123A) and angiotensinogen (AGT-M235T), are associated with CAD in the Saudi population. We recruited 225 subjects with angiographically confirmed CAD who had identical ethnic backgrounds and 110 control subjects. The polymerase chain reaction-restriction fragment length polymorphisms (RFLP) technique was used to detect polymorphisms in the RAS gene.
RESULTS
Within the CAD group, for the ACE I/D genotype, DD was found in 64.4%, 26.3% carried the ID genotype, and 9.3% carried the II genotype. Within the control group, the DD genotype was found in 56.4%, 23.6% carried the ID genotype, and 20% carried the II genotype. The odds ratio (OR) of the ACE DD vs II genotype with a 95% confidence interval (CI) was 2.45 (1.26-4.78), with p = 0.008. For the Ang II AT2 receptor C3123A genotype, within the CAD group, CC was found in 39.6%, 17.8% carried the CA genotype, and 42.6% carried the AA genotype. Within the control group, CC was found in 39.1%, 60.9% carried the CA genotype, and there was an absence of the AA genotype. The OR of the Ang II AT2 receptor C3123A CC vs AA genotypes (95% CI) was 0.01, with p = 0.0001. A significant association with CAD was shown. For the AGT-M235T genotype, within the CAD group, MM was found in 24.0%, 43.6% carried the MT genotype and 32.4% carried the TT genotype. Within the control group, MM was found in 26.4%, 45.5% carried the TT genotype and 28.2% carried the MT genotype. The OR of MM vs TT (95% CI) was 0.79 (0.43 to 1.46), which was insignificant.
CONCLUSIONS
There is an association between the ACE I/D and Ang II AT2 receptor C3123A polymorphisms and CAD, however, no association was detected between the AGT M235T polymorphism and CAD in the Saudi population.
背景
肾素 - 血管紧张素系统（RAS）各组分的多态性在部分个体冠状动脉疾病（CAD）的发生和发展中具有重要作用。我们本次研究的目的是确定肾素 - 血管紧张素系统的三个多态性，即血管紧张素转换酶插入/缺失（ACE I/D）、血管紧张素II受体2（Ang II AT2 - C3123A）和血管紧张素原（AGT - M235T）是否与沙特人群的CAD相关。我们招募了225名经血管造影证实患有CAD且种族背景相同的受试者以及110名对照受试者。采用聚合酶链反应 - 限制性片段长度多态性（RFLP）技术检测RAS基因中的多态性。
结果
在CAD组中，对于ACE I/D基因型，64.4%为DD型，26.3%为ID型，9.3%为II型。在对照组中，56.4%为DD基因型，23.6%为ID基因型，20%为II基因型。ACE DD型与II型基因型的优势比（OR）及95%置信区间（CI）为2.45（1.26 - 4.78），p = 0.008。对于血管紧张素II受体2（Ang II AT2）C3123A基因型，在CAD组中，39.6%为CC型，17.8%为CA型，42.6%为AA型。在对照组中，39.1%为CC型，60.9%为CA型，且无AA型。血管紧张素II受体2（Ang II AT2）C3123A CC型与AA型基因型的OR（95%CI）为0.01，p = 0.0001。显示出与CAD有显著关联。对于AGT - M235T基因型，在CAD组中，24.0%为MM型，43.6%为MT型，32.4%为TT型。在对照组中，26.4%为MM型，45.5%为TT型，28.2%为MT型。MM型与TT型的OR（95%CI）为0.79（0.43至1.46），无统计学意义。
结论
ACE I/D和血管紧张素II受体2（Ang II AT2）C3123A多态性与CAD之间存在关联，然而，在沙特人群中未检测到AGT M235T多态性与CAD之间的关联。
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