Fishleder A J
Cleve Clin J Med. 1989 Oct;56(7):716-21. doi: 10.3949/ccjm.56.7.716.
Recent studies have significantly broadened understanding of immunoglobulin production and T-cell receptor formation at the gene level. A limited number of genes can be rearranged during the course of B-cell or T-cell development to yield unique DNA sequences that will code for specific antibodies and T-cell receptor proteins. Southern blot hybridization analysis allows sensitive examination of lymphocyte DNA for the presence or absence of gene rearrangements. General mechanisms that underlie immunoglobulin and T-cell receptor rearrangement are reviewed, along with the diagnostic applications of detection of gene rearrangement by Southern blot hybridization technique.
最近的研究显著拓宽了在基因水平上对免疫球蛋白产生和T细胞受体形成的理解。在B细胞或T细胞发育过程中，有限数量的基因可以重排，以产生将编码特定抗体和T细胞受体蛋白的独特DNA序列。Southern印迹杂交分析能够灵敏地检测淋巴细胞DNA中基因重排的存在与否。本文综述了免疫球蛋白和T细胞受体重排的一般机制，以及通过Southern印迹杂交技术检测基因重排的诊断应用。
