Ge Baoyu, Li Zhigang, Yang Lingling, Wang Ruiyong, Chang Junbiao
Department of Chemistry, Zhengzhou University, Zhengzhou, China.
Department of Chemistry, Zhengzhou University, Zhengzhou, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2015;149:667-73. doi: 10.1016/j.saa.2015.04.106. Epub 2015 May 8.
CdTe quantum dots (QDs) were synthesized in aqueous solution using thioglycolic acid (TGA) as stabilizing agents. The interaction between TGA-CdTe QDs and fat mass and obesity-associated (FTO) protein was investigated by fluorescence, UV-visible absorption, synchronous fluorescence and three-dimensional fluorescence spectroscopy. Results revealed that TGA-CdTe QDs could strongly quench the intrinsic fluorescence of FTO protein with a static quenching procedure. Both the van der Waals and hydrogen bonding played a major role in stabilizing the complex. The binding constant and thermodynamic parameters at different temperatures were obtained. In addition, we found that the fluorescence intensity of QDs was significantly enhanced by the addition of FTO protein. Based on this, a sensitive method for detecting FTO protein was obtained in the linear range of 5.52×10(-9)-6.62×10(-7) mol L(-1) with the detection limit of 1.14×10(-9) mol L(-1). The influences of factors on the interaction between FTO protein and TGA-CdTe QDs were studied.
采用巯基乙酸（TGA）作为稳定剂，在水溶液中合成了碲化镉量子点（QDs）。通过荧光光谱、紫外 - 可见吸收光谱、同步荧光光谱和三维荧光光谱研究了TGA - CdTe量子点与脂肪量和肥胖相关（FTO）蛋白之间的相互作用。结果表明，TGA - CdTe量子点能够通过静态猝灭过程强烈猝灭FTO蛋白的固有荧光。范德华力和氢键在稳定复合物中都起主要作用。获得了不同温度下的结合常数和热力学参数。此外，我们发现添加FTO蛋白后量子点的荧光强度显著增强。基于此，建立了一种检测FTO蛋白的灵敏方法，线性范围为5.52×10(-9)-6.62×10(-7) mol L(-1)，检测限为1.14×10(-9) mol L(-1)。研究了各种因素对FTO蛋白与TGA - CdTe量子点之间相互作用的影响。
