Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
J Photochem Photobiol B. 2015 Jul;148:262-267. doi: 10.1016/j.jphotobiol.2015.04.028. Epub 2015 May 8.
Determination of the superoxide dismutase activity is an important issue in the fields of biochemistry and the medical sciences. In the riboflavin/nitro blue tetrazolium (B2/NBT) method, the light sources used for generating superoxide anion radicals from light-excited riboflavin are normally fluorescent lamps. However, the conditions of B2/NBT experiments vary. This study investigated the effect of the light source on the light-excitation of riboflavin. The effectiveness of the photolysis was controlled by the wavelength of the light source. The spectra of fluorescent lamps are composed of multiple colour lights, and the emission spectra of fluorescent lamps made by different manufacturers may vary. Blue light was determined to be the most efficient for the photochemical reaction of riboflavin in visible region. The quality of the blue light in fluorescent lamps is critical to the photo-decomposition of riboflavin. A blue light is better than a fluorescent lamp for the photo-decomposition of riboflavin. The performance of the B2/NBT method is thereby optimized.
超氧化物歧化酶活性的测定在生物化学和医学科学领域是一个重要的问题。在核黄素/氮蓝四唑（B2/NBT）法中，用于从光激发核黄素生成超氧阴离子自由基的光源通常是荧光灯。然而，B2/NBT 实验的条件会有所不同。本研究探讨了光源对核黄素光激发的影响。光解的效果受光源波长的控制。荧光灯的光谱由多种颜色的光组成，不同制造商生产的荧光灯的发射光谱可能会有所不同。蓝光被确定为可见光区域核黄素光化学反应最有效的光。荧光灯中蓝光的质量对核黄素的光解至关重要。与荧光灯相比，蓝光更有利于核黄素的光解。从而优化了 B2/NBT 方法的性能。
