Department of Biotechnology, Ming-Chuan University, Taoyuan 33343, Taiwan.
Department of Science Education and Application, National Taichung University of Education, Taichung 40306, Taiwan.
J Photochem Photobiol B. 2017 Sep;174:355-363. doi: 10.1016/j.jphotobiol.2017.08.018. Epub 2017 Aug 12.
Crystal violet (CV) is applied in daily use mainly as a commercial dye and antimicrobial agent. Waste water containing CV may affect aquatic ecosystems. Riboflavin, also known as vitamin B, is non-toxic and an essential vitamin required for the functions of the human body. Riboflavin is photosensitive to UV and visible light in terms of generating reactive oxygen species. This study investigated the potential application of blue light on riboflavin, so as to come up with an effective way of degrading CV during its treatment. Photosensitivity of CV leading to degradation in the presence of riboflavin was investigated by light intensity, exposure time, and irradiation dosage. The degradation of CV during riboflavin photolysis treatment was studied by a UV/vis spectrometry and chromatography. The effects of CV degradation on microbial viability are relevant when considering the influences on the ecosystem. This study proved that riboflavin photochemical treatment with blue light degrades CV dye by ROS formation. The riboflavin photolysis-treated CV solution appeared to be transparent during conformational transformations of the CV that was rearranged by free radical species generated from riboflavin photolysis. After riboflavin photolysis, colony-forming units (CFUs) were determined for each CV solution. CFU preservation was 85.2% for the CV dissolved riboflavin solution treated with blue light irradiation at 2.0mW/cm for 120min. Degradation of CV by riboflavin photochemical procedures can greatly reduce antimicrobial ability and serve as an environmental friendly waste water treatment method. Our results presented here concerning riboflavin photolysis in degradation of CV provide a novel technique, and a simple and safe practice for environmental decontamination processes.
结晶紫 (CV) 主要用作商业染料和抗菌剂，在日常生活中得到广泛应用。含有 CV 的废水可能会影响水生生态系统。核黄素，又称维生素 B，是无毒的，是人体功能所必需的维生素。核黄素在生成活性氧方面对紫外线和可见光具有光敏性。本研究探讨了蓝光在核黄素中的潜在应用，以期在处理 CV 时找到一种有效的降解方法。通过光强、暴露时间和辐照剂量研究了 CV 在核黄素存在下的光降解。通过 UV/vis 光谱法和色谱法研究了 CV 在核黄素光解处理过程中的降解情况。在考虑对生态系统的影响时，CV 降解对微生物生存能力的影响是相关的。本研究证明，蓝光下的核黄素光化学处理通过 ROS 形成来降解 CV 染料。在 CV 的构象转变过程中，CV 溶液看起来是透明的，这是由于核黄素光解产生的自由基物种对 CV 进行了重排。在核黄素光解后，对每种 CV 溶液的集落形成单位 (CFU) 进行了测定。用蓝光照射，在 2.0mW/cm 下辐照 120min 处理 CV 溶解核黄素溶液时，CFU 保存率为 85.2%。核黄素光化学处理 CV 的降解可以大大降低其抗菌能力，是一种环保的废水处理方法。我们在这里介绍的有关 CV 降解的核黄素光解的结果提供了一种新颖的技术，为环境净化过程提供了一种简单、安全的实践方法。
