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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

McMurtry J P, Steele N C

USDA-Agricultural Research Service, Beltsville Agricultural Research Center, MD 20705.

Comp Biochem Physiol B. 1989;94(1):49-51. doi: 10.1016/0305-0491(89)90009-6.

A sequential double antibody radioimmunoassay (RIA) has been developed using synthetic chicken calcitonin (CT) as antigen, tracer and standard. 2. The immunoassay has a minimum detection limit of 0.5 ng and effective dose (ED50) of 7 ng. Serial dilutions of chicken and turkey plasma were parallel to serial dilutions of CT standard. Extracts of chicken and turkey ultimobranchial glands caused parallel displacement of tracer similar to synthetic CT. 3. Primary antisera (anti-chicken CT) was raised in guinea pigs immunized with RIBI: animals treated with Freund's complete adjuvant failed to respond. 4. Chicken CT was determined to have a half-life of 60 sec in the turkey hen. Development of a homologous RIA for avian CT will allow studies to elucidate the role of this hormone in birds.

已开发出一种连续双抗体放射免疫测定法（RIA），使用合成鸡降钙素（CT）作为抗原、示踪剂和标准品。2. 该免疫测定法的最低检测限为0.5纳克，有效剂量（ED50）为7纳克。鸡和火鸡血浆的系列稀释与CT标准品的系列稀释呈平行关系。鸡和火鸡鳃后腺提取物引起示踪剂的平行位移，类似于合成CT。3. 用RIBI免疫豚鼠制备了一级抗血清（抗鸡CT）：用弗氏完全佐剂处理的动物无反应。4. 已确定鸡CT在火鸡母鸡体内的半衰期为60秒。开发一种用于禽CT的同源RIA将有助于开展研究以阐明这种激素在鸟类中的作用。

McMurtry J P, Steele N C

USDA-Agricultural Research Service, Beltsville Agricultural Research Center, MD 20705.

Comp Biochem Physiol B. 1989;94(1):49-51. doi: 10.1016/0305-0491(89)90009-6.

A sequential double antibody radioimmunoassay (RIA) has been developed using synthetic chicken calcitonin (CT) as antigen, tracer and standard. 2. The immunoassay has a minimum detection limit of 0.5 ng and effective dose (ED50) of 7 ng. Serial dilutions of chicken and turkey plasma were parallel to serial dilutions of CT standard. Extracts of chicken and turkey ultimobranchial glands caused parallel displacement of tracer similar to synthetic CT. 3. Primary antisera (anti-chicken CT) was raised in guinea pigs immunized with RIBI: animals treated with Freund's complete adjuvant failed to respond. 4. Chicken CT was determined to have a half-life of 60 sec in the turkey hen. Development of a homologous RIA for avian CT will allow studies to elucidate the role of this hormone in birds.

已开发出一种连续双抗体放射免疫测定法（RIA），使用合成鸡降钙素（CT）作为抗原、示踪剂和标准品。2. 该免疫测定法的最低检测限为0.5纳克，有效剂量（ED50）为7纳克。鸡和火鸡血浆的系列稀释与CT标准品的系列稀释呈平行关系。鸡和火鸡鳃后腺提取物引起示踪剂的平行位移，类似于合成CT。3. 用RIBI免疫豚鼠制备了一级抗血清（抗鸡CT）：用弗氏完全佐剂处理的动物无反应。4. 已确定鸡CT在火鸡母鸡体内的半衰期为60秒。开发一种用于禽CT的同源RIA将有助于开展研究以阐明这种激素在鸟类中的作用。