Kwok C F, Jap T S, Ho L T
Zhonghua Yi Xue Za Zhi (Taipei). 1989 Jan;43(1):9-14.
A sensitive sequential radioimmunoassay of calcitonin (CT) is described. Synthetic human CT was used for 125I-labelling and for preparation of anti-sera. The labelled hormone was purified by QUSO and P10 column. The specific activity of the purified 125I-CT was 39.8 Ci/g. The anti-sera was produced by immunization of guinea pigs with human synthetic CT which was conjugated with carbodiimide onto BSA (bovine serum albumin). Separation of free from antibody bound 125I-labelled CT was performed using DCC precipitation. Standard curves of extracted plasma, extracted plasma plus human CT were parallel to the standard curve. Recovery of 62.5 to 500 pg/ml CT were in the range of 80.72 to 113.4%. The Scatchart plot of the binding data showed a straight line with a kd of 56 pM and Bmax of 35 pM. The specificity of the antiserum was good. The lower limit of detection was 31.25 pg/ml. Our results indicate that this assay system may be useful in both clinical use and research.
本文描述了一种灵敏的降钙素（CT）序贯放射免疫测定法。合成人降钙素用于¹²⁵I标记和抗血清制备。标记激素通过QUSO和P10柱纯化。纯化后的¹²⁵I-CT比活为39.8 Ci/g。抗血清通过用人合成降钙素免疫豚鼠产生，该降钙素通过碳二亚胺与牛血清白蛋白（BSA）偶联。使用DCC沉淀法分离游离的和与抗体结合的¹²⁵I标记降钙素。提取血浆、提取血浆加人降钙素的标准曲线与标准曲线平行。62.5至500 pg/ml降钙素的回收率在80.72%至113.4%范围内。结合数据的Scatchart图显示为一条直线，解离常数（kd）为56 pM，最大结合容量（Bmax）为35 pM。抗血清的特异性良好。检测下限为31.25 pg/ml。我们的结果表明，该测定系统可能在临床应用和研究中都有用。
