源自人类多能干细胞的多能神经嵴细胞的诱导方法。
Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.
作者信息
Avery John, Dalton Stephen
机构信息
Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, Paul D. Coverdell Center for Biomedical and Health Sciences, University of Georgia, 500 DW Brooks Drive, Athens, GA, 30602, USA.
出版信息
Methods Mol Biol. 2016;1341:197-208. doi: 10.1007/7651_2015_234.
Abstract
Multipotent, neural crest cells (NCCs) produce a wide range of cell types during embryonic development. This includes melanocytes, peripheral neurons, smooth muscle cells, osteocytes, chondrocytes, and adipocytes. The protocol described here allows for highly efficient differentiation of human pluripotent stem cells to a neural crest fate within 15 days. This is accomplished under feeder-free conditions, using chemically defined medium supplemented with two small molecule inhibitors that block glycogen synthase kinase 3 (GSK3) and bone morphogenic protein (BMP) signaling. This technology is well suited as a platform to understand in greater detail the pathogenesis of human disease associated with impaired neural crest development/migration.
摘要
多能性神经嵴细胞（NCCs）在胚胎发育过程中产生多种细胞类型。这包括黑素细胞、外周神经元、平滑肌细胞、骨细胞、软骨细胞和脂肪细胞。本文所述方案可在15天内将人多能干细胞高效分化为神经嵴细胞命运。这是在无饲养层条件下完成的，使用添加了两种小分子抑制剂的化学成分确定的培养基，这两种抑制剂可阻断糖原合酶激酶3（GSK3）和骨形态发生蛋白（BMP）信号传导。这项技术非常适合作为一个平台，以更详细地了解与神经嵴发育/迁移受损相关的人类疾病的发病机制。
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