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钙化骨骺软骨“晶体残影”的组织化学特性

Histochemical properties of the "crystal ghosts" of calcifying epiphyseal cartilage.

作者信息

Bonucci E, Silvestrini G, di Grezia R

机构信息

Department of Human Biopathology, La Sapienza University, Rome.

出版信息

Connect Tissue Res. 1989;22(1-4):43-50; discussion 53-61.

PMID:2598670
Abstract

The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These results suggest that crystal ghosts are, or derive from, proteoglycans of calcifying matrix.

摘要

晶状幽灵代表一种与晶体相关的有机物质,它可被酸性磷钨酸、高碘酸-银甲胺和高碘酸-硫代氨基脲-锇染色,在pH 2.0时与阳离子和胶体铁发生反应,甲基化和皂化后无反应。这些结果表明,晶状幽灵是钙化基质的蛋白聚糖,或者源自钙化基质的蛋白聚糖。

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引用本文的文献

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Understanding nanocalcification: a role suggested for crystal ghosts.理解纳米钙化:晶体幽灵所起的作用。
Mar Drugs. 2014 Jul 23;12(7):4231-46. doi: 10.3390/md12074231.
2
Association of specific proteolytic processing of bone sialoprotein and bone acidic glycoprotein-75 with mineralization within biomineralization foci.骨唾液蛋白和骨酸性糖蛋白-75的特定蛋白水解加工与生物矿化灶内矿化的关联。
J Biol Chem. 2007 Sep 7;282(36):26002-13. doi: 10.1074/jbc.M701332200. Epub 2007 Jul 5.
3
Gene expression and immunohistochemical localization of biglycan in association with mineralization in the matrix of epiphyseal cartilage.
双糖链蛋白聚糖在骨骺软骨基质矿化过程中的基因表达及免疫组化定位
Histochem J. 2000 Mar;32(3):175-86. doi: 10.1023/a:1004099324169.
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X-ray diffraction, electron microscopy, and Fourier transform infrared spectroscopy of apatite crystals isolated from chicken and bovine calcified cartilage.从鸡和牛的钙化软骨中分离出的磷灰石晶体的X射线衍射、电子显微镜和傅里叶变换红外光谱分析。
Calcif Tissue Int. 1996 Jul;59(1):58-63. doi: 10.1007/s002239900086.
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Matrix vesicles and focal proteoglycan aggregates are the nucleation sites revealed by the lanthanum incubation method: a correlated study on the hypertrophic zone of the rat epiphyseal cartilage.基质小泡和局灶性蛋白聚糖聚集体是镧孵育法揭示的成核位点:对大鼠骨骺软骨肥大区的相关研究
Calcif Tissue Int. 1996 Apr;58(4):273-82. doi: 10.1007/BF02508648.