从意大利中部城市污水分离的维氏气单胞菌和嗜水气单胞菌中新型天然CphA金属β-内酰胺酶CphA4和CphA5的鉴定
Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy.
作者信息
Bottoni Carlo, Marcoccia Francesca, Compagnoni Chiara, Colapietro Martina, Sabatini Alessia, Celenza Giuseppe, Segatore Bernardetta, Maturo Maria Giovanna, Amicosante Gianfranco, Perilli Mariagrazia
机构信息
Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy
出版信息
Antimicrob Agents Chemother. 2015 Aug;59(8):4990-3. doi: 10.1128/AAC.00628-15. Epub 2015 May 18.
Abstract
Two new natural CphA metallo-β-lactamases, the CphA4 and CphA5 enzymes, were identified in water samples from municipal sewage in central Italy. Compared to CphA, the CphA4 and CphA5 enzymes showed numerous point mutations. These enzymes have a narrow spectrum of substrates focused on carbapenems only. CphA5 showed kcat values about 40-, 12-, and 97-fold higher than those observed for CphA4 versus imipenem, ertapenem, and biapenem, respectively.
摘要
在意大利中部城市污水的水样中鉴定出两种新的天然CphA金属β-内酰胺酶，即CphA4和CphA5酶。与CphA相比，CphA4和CphA5酶表现出许多点突变。这些酶的底物谱狭窄，仅集中于碳青霉烯类。相对于亚胺培南、厄他培南和美罗培南，CphA5的催化常数（kcat）值分别比CphA4高约40倍、12倍和97倍。
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本文引用的文献
1
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J Glob Antimicrob Resist. 2013 Dec;1(4):217-220. doi: 10.1016/j.jgar.2013.07.002. Epub 2013 Aug 20.
2
Aeromonas hydrophila and Aeromonas veronii predominate among potentially pathogenic ciprofloxacin- and tetracycline-resistant aeromonas isolates from Lake Erie.
Appl Environ Microbiol. 2014 Feb;80(3):841-8. doi: 10.1128/AEM.03645-13. Epub 2013 Nov 15.
3
Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption.
Int J Food Microbiol. 2012 Oct 15;159(3):230-9. doi: 10.1016/j.ijfoodmicro.2012.09.008. Epub 2012 Sep 23.
4
Aeromonas spp. clinical microbiology and disease.
J Infect. 2011 Feb;62(2):109-18. doi: 10.1016/j.jinf.2010.12.003. Epub 2010 Dec 14.
5
First occurrence of an IMP metallo-beta-lactamase in Aeromonas caviae: IMP-19 in an isolate from France.
Antimicrob Agents Chemother. 2007 Dec;51(12):4486-8. doi: 10.1128/AAC.01462-06. Epub 2007 Oct 15.
6
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J Mol Biol. 2005 Jan 28;345(4):785-95. doi: 10.1016/j.jmb.2004.10.070.
7
Kinetic and spectroscopic characterization of native and metal-substituted beta-lactamase from Aeromonas hydrophila AE036.
FEBS Lett. 2000 Feb 11;467(2-3):221-5. doi: 10.1016/s0014-5793(00)01102-9.
8
The Aeromonas metallo-beta-lactamases: genetics, enzymology, and contribution to drug resistance.
Microb Drug Resist. 1996 Summer;2(2):245-52. doi: 10.1089/mdr.1996.2.245.
9
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10
Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases.
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