Department of Food Science and Technology, Sari Agriculture and Natural Resources University Sari, Mazandaran, Iran.
Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University Tehran, Iran.
Food Sci Nutr. 2015 May;3(3):179-87. doi: 10.1002/fsn3.201. Epub 2015 Jan 21.
In this study, the bioactive compounds of loquat fruit (Eriobotrya japonica Lindl.) skin and pulp extracted by two extraction methods (solvent and ultrasound-assisted) with three solvents (ethanol, water and ethanol-water) were compared to supercritical fluid extraction. The antioxidant activities of skin and pulp extracts were evaluated and compared to tertiary butylhydroquinone (TBHQ) using 2, 2-diphenyl-1-picrylhydrazyl (DPPH˙) radical scavenging, β-carotene bleaching, and the Rancimat assays. In DPPH assay solvent extracts of skin by ethanol (SSE) and ethanol-water (SSEW) showed strong inhibitory activity. The SSEW also showed the highest inhibition percentage of 85.58% by the β-carotene bleaching assay and longest induction time of 4.78 h by the Rancimat method. The large amount of tocopherols and phenolics contained in the skin extract may cause its strong antioxidant ability. The results indicated that the solvent extraction with ethanol-water produced the maximum extraction yield of phenolic and tocopherol compounds from loquat fruit skin and pulp. Furthermore, solvent extraction was the most effective in antioxidant activity of the extracts compared to other extraction techniques.
本研究比较了溶剂提取法（溶剂法和超声辅助法）和三种溶剂（乙醇、水和乙醇-水）与超临界流体提取法从枇杷果皮和果肉中提取的生物活性化合物。采用 2,2-二苯基-1-苦基肼（DPPH˙）自由基清除法、β-胡萝卜素漂白法和 Rancimat 法评价并比较了果皮和果肉提取物的抗氧化活性，并与叔丁基对苯二酚（TBHQ）进行了比较。在 DPPH 测定中，乙醇（SSE）和乙醇-水（SSEW）溶剂提取的果皮提取物表现出很强的抑制活性。β-胡萝卜素漂白法测定中，SSEW 的抑制率最高，为 85.58%；Rancimat 法测定中，诱导时间最长，为 4.78 h。果皮提取物中含有大量的生育酚和酚类物质，可能导致其具有较强的抗氧化能力。结果表明，乙醇-水溶剂提取法从枇杷果皮和果肉中提取了最大量的酚类和生育酚类化合物。此外，与其他提取技术相比，溶剂提取法对提取物的抗氧化活性最为有效。
