Simborio Hannah Leah Tadeja, Lee Jin Ju, Bernardo Reyes Alisha Wehdnesday, Hop Huynh Tan, Arayan Lauren Togonon, Min Wongi, Lee Hu Jang, Yoo Han Sang, Kim Suk
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
Microb Pathog. 2015 Jun-Jul;83-84:41-6. doi: 10.1016/j.micpath.2015.05.004. Epub 2015 May 16.
Currently, there are several serodiagnostic tools available for brucellosis, however, it is difficult to differentiate an active infection from vaccination. Hence, there is a great need to develop alternative means that can distinguish between these two conditions without utilizing lipopolysaccharide (LPS). This study was an attempt to determine the efficacy of combined recombinant Brucella (B.) abortus outer membrane proteins (rOmps) and individual rOmps in the serodiagnosis of brucellosis by enzyme linked immunosorbent assay (ELISA), utilizing both that standard tube agglutination test (TAT)-positive and -negative serum samples from Korean native cattle. The results are very interesting and promising because the combined rOmp antigens used in the study were highly reactive with the TAT-positive serum samples. The combined rOmps sensitivity, specificity and accuracy were 215/232 (92.67%), 294/298 (98.66%) and 509/530 (96.04%), respectively. While these results are preliminary, the tests performed have very high potential in the serodiagnosis of brucellosis and likewise, the combined rOmps can be used for future vaccine production.
目前，有几种用于布鲁氏菌病的血清学诊断工具，然而，很难区分是活动性感染还是接种疫苗所致。因此，迫切需要开发无需利用脂多糖（LPS）就能区分这两种情况的替代方法。本研究试图通过酶联免疫吸附测定（ELISA），利用韩国本土牛标准试管凝集试验（TAT）阳性和阴性血清样本，来确定重组布鲁氏菌流产亚种外膜蛋白（rOmps）组合及单个rOmps在布鲁氏菌病血清学诊断中的效力。结果非常有趣且很有前景，因为研究中使用的rOmp抗原组合与TAT阳性血清样本反应性很高。rOmps组合的敏感性、特异性和准确性分别为215/232（92.67%）、294/298（98.66%）和509/530（96.04%）。虽然这些结果是初步的，但所进行的检测在布鲁氏菌病血清学诊断中具有很高的潜力，同样，rOmps组合可用于未来的疫苗生产。
