Key Lab of Environment and Health, School of Public Health, Xuzhou Medical University, Xuzhou, 221004, China.
Department of Infection Control, The First Hospital of Jilin University, Changchun, 130021, China.
Biochem Biophys Res Commun. 2021 Jun 30;560:126-131. doi: 10.1016/j.bbrc.2021.04.127. Epub 2021 May 11.
Brucellosis has placed a heavy economic burden on numerous countries and has consumed considerable medical resources worldwide. To improve the specificity and sensitivity of serological methods for diagnosing brucellosis, it is important to develop new diagnostic antigens. Brucella outer membrane proteins(omps) possess good immunogenicity, but there is a scarcity of comparative studies of these proteins in the clinical diagnosis of brucellosis. In this study, six recombinant Brucella outer membrane proteins, omp10, omp16, omp19, omp25, omp31 and BP26, were expressed in prokaryotic cells and utilized as diagnostic antigens. The clinical sera of humans, bovines and goats with brucellosis were analyzed by indirect ELISA using these proteins, lipopolysaccharide(LPS) and Rose Bengale Ag, served as positive-control antigens. In diagnosing human and goat serum, BP26 exhibited the highest diagnostic accuracy of 96.45% and 95.00%, respectively, while omp31 exhibited the strongest ability to detect Brucella in bovine serum with an accuracy of 84.03%. Cross-reaction experiments also confirmed that the diagnostic specificities of omp31 and BP26 were higher than those of the LPS and Rose Bengale Ag antigens. The results of this study indicate that omp31 and BP26 are candidate antigens with high potential application value in the clinical diagnosis of brucellosis.
布鲁氏菌病给许多国家带来了沉重的经济负担，并消耗了全球大量的医疗资源。为了提高血清学方法诊断布鲁氏菌病的特异性和敏感性，开发新的诊断抗原非常重要。布鲁氏菌外膜蛋白（omps）具有良好的免疫原性，但这些蛋白在布鲁氏菌病的临床诊断中比较研究较少。本研究在原核细胞中表达了 6 种重组布鲁氏菌外膜蛋白 omp10、omp16、omp19、omp25、omp31 和 BP26，并将其作为诊断抗原用于间接 ELISA 分析。用这些蛋白、脂多糖（LPS）和 Rose Bengale Ag 作为阳性对照抗原，分析了人类、牛和羊布鲁氏菌病的临床血清。在诊断人血清和羊血清时，BP26 的诊断准确性最高，分别为 96.45%和 95.00%，而 omp31 检测牛血清中布鲁氏菌的能力最强，准确性为 84.03%。交叉反应实验也证实，omp31 和 BP26 的诊断特异性高于 LPS 和 Rose Bengale Ag 抗原。本研究结果表明，omp31 和 BP26 是具有高应用潜力的候选抗原，可用于布鲁氏菌病的临床诊断。
