Battula Kiran Kumar, Nappanveettil Giridharan, Nakanishi Satoshi, Kuramoto Takashi, Friedman Jeffry M, Kalashikam Rajender Rao
Molecular Genetics Laboratory, National Centre for Laboratory Animal Science (NCLAS), National Institute of Nutrition (NIN), Tarnaka, Hyderabad, 500007, India.
Biochem Genet. 2015 Jun;53(4-6):132-40. doi: 10.1007/s10528-015-9679-8. Epub 2015 May 20.
WNIN (Wistar/NIN) is an inbred rat strain maintained at National Institute of Nutrition (NIN) for more than 90 years, and WNIN/Ob is an obese mutant originated from it. To determine their genetic relatedness with major rat strains in biomedical research, they were genotyped at various marker loci. The recently identified markers for albino and hooded mutations which clustered all the known albino rats into a single lineage also included WNIN and WNIN/Ob rats. Genotyping using microsatellite DNA markers and phylogenetic analysis with 49 different rat strains suggested that WNIN shares a common ancestor with many Wistar originated strains. Fst estimates and Fischer's exact test suggest that WNIN rats differed significantly from all other strains tested. WNIN/Ob though shows hyper-leptinemia, like Zucker fatty rat, did not share the Zucker fatty rat mutation. The above analyses suggest WNIN as a highly differentiated rat strain and WNIN/Ob a novel obese mutant evolved from it.
WNIN（Wistar/NIN）是一种在国家营养研究所（NIN）维持了90多年的近交系大鼠品系，WNIN/Ob是源自该品系的肥胖突变体。为了确定它们与生物医学研究中主要大鼠品系的遗传相关性，对它们在多个标记位点进行了基因分型。最近鉴定出的白化和头巾突变标记将所有已知的白化大鼠归为一个单一谱系，其中也包括WNIN和WNIN/Ob大鼠。使用微卫星DNA标记进行基因分型以及对49种不同大鼠品系进行系统发育分析表明，WNIN与许多源自Wistar的品系有共同的祖先。Fst估计值和费舍尔精确检验表明，WNIN大鼠与所有其他测试品系有显著差异。WNIN/Ob虽然像Zucker肥胖大鼠一样表现出高瘦素血症，但并未共享Zucker肥胖大鼠的突变。上述分析表明WNIN是一个高度分化的大鼠品系，WNIN/Ob是由此进化而来的新型肥胖突变体。
