Göhring Holger, Paulus Michael, Salmen Paul, Wirkert Florian, Kruse Theresa, Degen Patrick, Stuhr Susan, Rehage Heinz, Tolan Metin
Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
J Phys Condens Matter. 2015 Jun 17;27(23):235103. doi: 10.1088/0953-8984/27/23/235103. Epub 2015 May 20.
A study of lysozyme adsorption below a behenic acid membrane and at the solid-liquid interface between aqueous lysozyme solution and a silicon wafer in the presence of sodium chloride is presented. The salt concentration was varied between 1 mmol L(-1) and 1000 mmol L(-1). X-ray reflectivity data show a clear dependence of the protein adsorption on the salt concentration. Increasing salt concentrations result in a decreased protein adsorption at the interface until a complete suppression at high concentrations is reached. This effect can be attributed to a reduced attractive electrostatic interaction between the positively charged proteins and negatively charged surfaces by charge screening. The measurements at the solid-liquid interfaces show a transition from unoriented order of lysozyme in the adsorbed film to an oriented order with the short protein axis perpendicular to the solid-liquid interface with rising salt concentration.
本文介绍了在山嵛酸膜下方以及在氯化钠存在的情况下，溶菌酶水溶液与硅片之间的固液界面处溶菌酶吸附的研究。盐浓度在1 mmol L⁻¹至1000 mmol L⁻¹之间变化。X射线反射率数据表明蛋白质吸附明显依赖于盐浓度。盐浓度增加导致界面处蛋白质吸附减少，直至在高浓度下完全抑制。这种效应可归因于通过电荷屏蔽减少了带正电的蛋白质与带负电表面之间有吸引力的静电相互作用。在固液界面处的测量表明，随着盐浓度的升高，吸附膜中溶菌酶从不规则排列转变为短蛋白质轴垂直于固液界面的规则排列。
