Grup de Biotecnologia Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Rambla Sant Nebridi 22, Terrassa 08222, Spain.
Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Barcelona, Bellaterra, Spain.
Ultrason Sonochem. 2024 Dec;111:107131. doi: 10.1016/j.ultsonch.2024.107131. Epub 2024 Oct 28.
The wearing of contact lenses (CLs) may cause bacterial infections, leading in turn to more serious complications and ultimately vision impairment. In this scenario, the first step is the adhesion of tear proteins, which provide anchoring points for bacterial colonization. A possible solution is the functionalization with an antimicrobial coating, though the latter may also lead to sight obstruction and user discomfort. In this study, adipic acid dihydrazide-modified hyaluronic acid-cobalt (II) (HA-ADH-Co) nanogels (NGs) were synthesized and deposited onto commercial CLs in a single-step sonochemical process. The coating hindered up to 60 % the protein adsorption and endowed the CLs with strong antibacterial activity against major ocular pathogens like Staphylococcus aureus and Pseudomonas aeruginosa, reducing their concentration by around 3 logs. Cytotoxicity assessment with human corneal cells demonstrated viabilities above 95 %. The nanocomposite coating did not affect the optical power and the light transmission of the CLs and provided enhanced wettability, important for the wearer comfort.
隐形眼镜（CL）的佩戴可能会导致细菌感染，进而导致更严重的并发症，最终导致视力受损。在这种情况下，第一步是泪蛋白的黏附，它为细菌定植提供了附着点。一种可能的解决方案是用抗菌涂层进行功能化，尽管后者也可能导致视力障碍和使用者不适。在这项研究中，己二酰肼修饰透明质酸-钴（II）（HA-ADH-Co）纳米凝胶（NG）被合成并通过一步超声化学过程沉积在商业隐形眼镜上。该涂层阻碍了高达 60%的蛋白质吸附，并使隐形眼镜对主要眼部病原体（如金黄色葡萄球菌和铜绿假单胞菌）具有强大的抗菌活性，将其浓度降低了约 3 个对数级。用人眼角膜细胞进行的细胞毒性评估显示，细胞活力超过 95%。纳米复合材料涂层不影响隐形眼镜的光学功率和光传输，并提供了增强的润湿性，这对佩戴者的舒适度很重要。
